@article{Лещук_Хареба_Присяжнюк_Шитікова_Стариченко_2020, title={Application of EST-SSR markers for analysis of polymorphism of lettuce varieties (Lactuca sativa L.) of domestic breeding}, volume={16}, url={https://journal.sops.gov.ua/article/view/209259}, DOI={10.21498/2518-1017.16.2.2020.209259}, abstractNote={<p class="a"><strong><span lang="EN-US">Purpose.</span></strong><span lang="EN-US"> Determination of molecular genetic polymorphism of lettuce cultivars of Ukrainian breeding by EST-SSR markers. </span></p><p class="a"><span lang="EN-US"><strong>Methods.</strong> Molecular genetic analysis, statistical methods. </span></p><p class="a"><span lang="EN-US"><strong>Results. </strong>The results of molecular genetic polymorphism study of 7 lettuce cultivars by 7 EST-SSR markers are presented. As a result of the analysis by studied markers, 37 alleles were detected with an average 5.29 alleles per locus. <span class="tlid-translationtranslation">The KSL-92 marker, which identified 7 alleles, </span>proved<span class="tlid-translationtranslation"> to be the most polymorphic </span>(PIC – Polymorphism Information Content 0.98). The lowest PIC value (0.57) was noted for the KSL-37 marker by which 3 alleles were identified. For assessing the genetic diversity of lettuce cultivars by EST-SSR markers, genetic distances between cultivars were determined based on Jaccard’s similarity coefficient. It was determined that the most similar cultivars with genetic distances 0.17 were ‘Zorepad’ and ‘Malakhit’, ‘Malakhit’ and ‘Dublianskyi’, ‘Dublianskyi’ and ‘Smuhlianka’, ‘Krutianskyi’ and ‘Smuhlianka’. The most distant cultivar was ‘Skarb’ with a genetic distance 0.00 compared to other studied cultivars. According to the calculated genetic distances, the cultivars ‘Zorepad’ and ‘Malakhit’, ‘Malakhit’ and Dublianskyi’, which belong to the same variety <em>Lactuca sativa</em> L. var. <em>secalina</em> have a strong genetic similarity. The cultivars‘Skarb’ and ‘Pohonych’<span class="tlid-translationtranslation">, which belong to the varieties <em>Lactuca sativa</em> L. var. <em>longifolia</em> and <em>Lactuca sativa</em> L. var. <em>angustana</em>, respectively, were genetically distant, what was confirmed by </span><em>genetic distances values</em><span class="tlid-translationtranslation"><em>.</em></span> It was determined that the Shannon index within the lettuce variety by 7 EST-SSR markers is 0.61, between varieties – 0.96, the average value is 1.57. </span></p><p class="a"><span lang="EN-US"><strong>Conclusions.</strong> According to the results of studies of 7 lettuce cultivars, it was found that the highest polymorphism was determined by the KSL-92 marker, the minimum of alleles number (3 alleles) was identified by KSL-37 marker. Based on calculated genetic distances, it was noticed that lettuce cultivars, genetic distances between which are 0.17, were the most similar. The most distant cultivar based on 7 EST-SSR markers was ‘Skarb’ cultivar.</span></p>}, number={2}, journal={Plant varieties studying and protection}, author={Лещук, Н. В. and Хареба, О. В. and Присяжнюк, Л. М. and Шитікова, Ю. В. and Стариченко, Є. М.}, year={2020}, month={Aug.}, pages={226–233} }