TY - JOUR AU - Гончаров, Ю. О. AU - Сатарова, Т. М. AU - Дзюбецький, Б. В. AU - Черчель, В. Ю. PY - 2016/12/28 Y2 - 2024/03/29 TI - Allelic status of key genes of carotenogenesis on DNA-markers in maize lines and their hybrids JF - Plant varieties studying and protection JA - PVSandP VL - IS - 4(33) SE - GENETICS DO - 10.21498/2518-1017.4(33).2016.88666 UR - https://journal.sops.gov.ua/article/view/88666 SP - 26-32 AB - <p style="text-indent: 0.4cm; margin-bottom: 0cm; line-height: 0.39cm; widows: 2; orphans: 2;" lang="en-US" align="JUSTIFY"><span style="color: #000000;"><span><span style="font-size: small;"><strong>Purpose. </strong>The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-<span>ε</span>-cyclase<em> (lcy</em><span><em>ε</em></span><em>) </em>and gene of <span>β</span>-carotene hydroxylase<em> (crtRB1)</em> for DNA-markers in domestic maize lines and their hybrids. </span></span></span></p><p style="text-indent: 0.4cm; margin-bottom: 0cm; line-height: 0.39cm; widows: 2; orphans: 2;" lang="en-US" align="JUSTIFY"><span style="color: #000000;"><span><span style="font-size: small;"><strong>Methods</strong>. DNA isolation, PCR, gel electrophoresis. </span></span></span></p><p style="text-indent: 0.4cm; margin-bottom: 0cm; line-height: 0.39cm; widows: 2; orphans: 2;" lang="en-US" align="JUSTIFY"><span style="color: #000000;"><span><span style="font-size: small;"><strong>Results.</strong> Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hyb­rids has been detected in gene of <span>β</span>-carotene hydroxylase for marker <em>crtRB1</em>-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-<span>ε</span>-cyclase <em>lcy</em><span>ε</span>-3’INDL and <em>lcy</em><span><em>ε</em></span><em>-</em>SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S<span><span lang="ru-RU">×</span></span>DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activi­ty of <span>β</span>-carotene hydroxylase owing to the mutation of gene <em>crtRB1</em> under the influence of transposone element at the 3’-end, the inhibition of <span>β</span>-carotene transition into <span>β</span>-cryptoxanthin can be expected, that allows to predict <span>β</span>-carotene accumulation in grain. <strong></strong></span></span></span></p><p style="text-indent: 0.4cm; margin-bottom: 0cm; line-height: 0.39cm; widows: 2; orphans: 2;" lang="en-US" align="JUSTIFY"><span style="color: #000000;"><span><span style="font-size: small;"><strong>Conclusions.</strong> The study of allelic status of carotenegenesis gene of lycopene-<span>ε</span>-cyclase in maize showed no polymorphism for markers <em>lcy</em><span><em>ε</em></span><em>-</em>3’INDL and <em>lcy</em><span><em>ε</em></span><em>-</em>SNP216 in eight inbred lines and their single crosses, along with this, for marker <em>lcy</em><span><em>ε</em></span><em>-</em>3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of <span>β</span>-carotene. For marker <em>crtRB1-</em>3’TE of gene of <span>β</span>-carotene hydroxylase, the studied breeding material was polymorphic. Allele of <em>crtRB1 </em>being favorable for the accumulation of <span>β</span>-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S<span><span lang="ru-RU">×</span></span>DK253ZSZM’, ‘DK272S<span><span lang="ru-RU">×</span></span>DK633/266zS,zM’ and ‘DK231S<span><span lang="ru-RU">×</span></span>DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of <span>β</span>-carotene hydroxylase<em> </em>codominantly.</span></span></span></p> ER -