Plant varieties studying and protection

Selection evaluation of new selfpollinated sunflower (Helianthus annuus L.) lines with resistance to sulfonylurea herbicides and downy mildew [Plasmopara halstedii (Farl.) Berl. et. de Tony]

2024-03-18T11:15:38+02:00

Purpose. To determine the breeding value of a new sunflower source with complex resistance to sulfonylurea herbicides and downy mildew. Methods. In the research process, field (hybridization, line testing, individual selection, line evaluation), visual (phenological observations), laboratory (immunological evaluation of resistance to DM), vegetation (evaluation of resistance to herbicides) and mathematical and statistical (processing of experimental data and determination of reliability of research results) methods were used. Results. During 2020–2023, new selfpollinated sunflower lines were studied in the crosspollination and breeding department of the Plant Breeding & Genetics Institute – National Center of Seeds and Cultivar Investigation (PBGI – NCSCI). Based on the results of the work, 33 selfpollinated sunflower lines with complex resistance to sulfonylurea herbicides and downy mildew (DM) were created and evaluated. The lines were created using domestic breeding populations that were able to reach their full genetic potential in various conditions. These populations were adapted to cultivation in the southern steppe of Ukraine and were resistant to a complex of diseases and pests. Additionally, they had increased seed yield and plasticity. The new source material obtained is constant, stably productive lines used in the subsequent breeding programme. According to the results of the trials, almost all the hybrids obtained (F₁) showed a yield of more than 1.0 t/ha. Lines with the highest level of combining ability in terms of yield (heterozygous hybrid progeny with increased viability for the main economic and valuable traits) will be selected for further research and will be involved in the creation of new hybrids resistant to sulfonylurea herbicides and DM. Conclusions. Research showed that traits such as sulfonylurea herbicide resistance and downy mildew resistance can be combined in one line. Herbicide resistance in sunflowers is easy to control in the field, while downy mildew resistance needs to be controlled in the laboratory.

Evaluation of the productivity potential of eggplant lines developed on the basis of interspecific hybridisation and gametic breeding

2024-03-18T11:05:53+02:00

Purpose. Improvement of the gene pool of the cultivated form of eggplant (Solanum melongena L.) through interspecific hybridisation with Solanum aethiopicum L. and gametic breeding; analysis of the interspecific lines for a set of valuable quantitative traits determining the yield structure. Methods. Studies for the comprehensive evaluation of eggplant lines of interspecific origin were carried out during 2021-2023 in protected soil conditions at the experimental base of the Institute for Vegetable and Melon Growing of the National Academy of Agrarian Sciences. Phenological observations and biometric measurements of plants of four lines were carried out. The stability of the manifestation of seven quantitative traits during the research years was determined by the methods of variation statistics (calculation of the mean square deviation (σ), coefficient of variation (V), Pearson's paired linear correlation (r_p)). Results. The analysis identified eggplant lines of interspecific origin that were superior to the standard variety 'Almaz' in terms of the level and stability of quantitative traits. Thus, F₆(Pavlotas-20/var. 'Almaz')I₁ and ВС₁[F₅ (Pavlotas-20/var. 'Almaz')]I₁ showed a statistically significant increase in fruit width (by 16.48% and 14.85%, respectively). Meanwhile, line BC₂ [F₅ (Pavlotas-20/var. 'Almaz')]I₁ exhibited higher productivity (by 18.11%) compared to the standard variety. The correlation analysis shows that the trait “Productivity of one plant” has a strong positive relationship with the trait “Number of fruits per plant” (r_p = 0.75) and a strong negative relationship with “Average fruit width” (r_p = -0.70) and “Duration of the period from mass germination to technical fruit maturity” (r_p = -0.72). The selected line BC₂[F₅(Pavlotas-20 / var. 'Almaz')]I₁ exhibited a four-day shorter period from mass germination to technical fruit maturity compared to the standard variety. Conclusions. The line BC₂[F₅(Pavlotas-20 / var. ' Almaz ')]I₁was isolated based on the complex of quantitative traits. It underwent an additional stage of gametophytic breeding, resulting in a high adaptive potential to growing conditions.

Selection of pollinators for promising largefruited varieties of sweet cherry Cerasus avium (L.) Moenh in ForestSteppe conditions

2024-02-14T12:58:10+02:00

Purpose. Selection of effective pollinators for promising largefruited sweet cherry varieties in the ForestSteppe of Ukraine. Methods. Field, laboratory and analytical methods were used in the research. Pollen viability and selffertility were determined in 29 varieties of different ripening periods. Pollinators were selected for the group of mediumlate and late varieties (‘Krupnoplidna’, ‘Talisman’, ‘Stark Hardy Giant’, ‘Anons’, ‘Temporion’, ‘Zodiak’, ‘Annushka’, ‘Anshlah’ and ‘Regina’). The surveys were carried out in accordance with the approved scientific and methodological recommendations. Results. In the group of early varieties, the highest pollen quality (77.9%) was produced by ‘Valerii Chkalov’; among the varieties of medium ripening period – ‘Talisman’ (control) (87.3%), ‘Yaroslavna’ (78.8%) and ‘Melitopolska Myrna’ (86.7%); medium late and late – ‘Drogan’s grosse gelbe’, ‘Kordia’, ‘Regina’, ‘Zodiac’ and ‘Annushka’ (80.9–88.0%). A full crop of all the varieties studied can only be guaranteed under the condition of crosspollination. The highest level of fruit set (47.9–56.1%) in ‘Talisman’, ‘Temporion’, ‘Stark Hardy Giant’ and ‘Anshlah’ was due to pollen of ‘Krupnoplidna’. Conclusions. The versatility of ‘Donchanka’ as a pollinator was revealed. When it was used, fruit set in relation to free pollination was 111.2–148.8%. ‘Krupnoplidna’ and ‘Stark Hardy Giant’ proved to be mutual pollinators and effective pollinators for the vast majority of varieties. The variety ‘Regina’ was best pollinated by ‘Donchanka’ and ‘Drogan’s grosse gelbe’ with fruit set of 24–26.6% and 134.8–149.4% respectively compared to the control.

Morphology of generative organs of Morus alba L. cultivars introduced in the conditions of the Forest Steppe of Ukraine

2024-04-02T11:37:12+03:00

Purpose. The aim was to study the features of flowering and fruiting of the mulberry under conditions of the RightBank ForestSteppe of Ukraine. Methods. The research was conducted in the forest steppe zone at the experimental base of the Institute of Horticulture of the NAAS in 2021–2023. The subject was plant material of different cultivars of mulberry (Morus alba), propagated from seed progeny of the ‘Krupnoplidna’ variety; selffertile mulberry plants were used as a control. The morphological description of the structural elements of the reproductive organs and the study of pollen viability were carried out according to published methods. The fruiting of the breeding forms was evaluated on a fivepoint scale. Results. All plants tested were found to have the ability to germinate pollen. The most viable pollen (75.1%) was found in male breeding form No. 5 after 12 hours exposure and a sucrose concentration of 50%. Exposure for 24 hours caused a decrease in germination energy of up to 63.1%. The latter was lowest in the pollen grains of plants of the triploid sample at both sucrose concentrations used. The comparison of morphological characteristics and seed productivity (per fruit) of the breeding forms of M. alba showed the genetic diversity of plants of cultivars of the species, which is manifested in the difference of phenotypic characteristics due to the variability of the range of their expression. Conclusions. The highest rates of white mulberry pollen germination were obtained for breeding form No. 5 – 79.1 ± 0.36% (male specimen) with 25% concentration of sucrose in the nutrient medium. The number of fruits formed on twoyearold shoots was 21–91. The evaluation of the fruiting of the cultivars was 4–5 points, and their infructescences were 2.26–3.97 cm long (large). In general, the study of the morphology of the reproductive organs of the breeding forms of M. alba, each of which forms filled seeds with high germination energy (91–98%), showed the intactness of the reproductive functions of mulberry, which indicates the successful passage of the plants through all stages of organogenesis.

Study on the stability of the productivity index of sorghum (Sorghum bicolor L.) varieties under ForestSteppe conditions

2024-01-22T11:13:13+02:00

Purpose. To determine the stability of seed productivity of sorghum (Sorghum bicolor L.) varieties. Methods. The research on common twocoloured grain sorghum varieties (‘Arabesk’, ‘Arkan’, ‘ES Villy’ and ‘Stepovyi’) was conducted in 2018–2020 in the ForestSteppe zone on the experimental fields of the Poltava, Vinnytsia and Cherkasy branches of the Ukrainian Institute for Plant Variety Examination (UIPVE). In accordance with the «Methodology for the examination of plant varieties of the group of cereals, grains and legumes for their suitability for distribution in Ukraine», trials were set up, material evaluated, phenological observations and biometric measurements of plants made and harvested. Seed quality indicators were determined in accordance with the “Methodology for the qualification examination of plant varieties for their suitability for distribution in Ukraine. Methods of quality determination”. The study used field, laboratory, biochemical and statistical methods. Results. Optimal conditions of the ForestSteppe ensured timely onset of phenological phases of plant growth and development and insignificant variation of vegetation period from 117 to 123 days. Productivity of sorghum was estimated by quantitative indicators of seed yield per plant and weight of 1000 seeds. Conclusions. The Levis coefficient C_S.F was calculated to characterise the phenotypic stability of varieties. The results showed that ‘ES Villy’ (1.00) and ‘Stepovyi’ (1.06) were stable in the experimental field of the Poltava branch of UIPVE. The conditions of Vinnytsia and Poltava branches were more favourable for the formation of high quality bicoloured sorghum grain and larger weight of 1000 seeds.

Yield and quality of winter wheat grain under different cultivation technologies

2024-03-18T11:26:37+02:00

Purpose. Study of the influence of preceding crops and sowing terms on the yield and quality of winter wheat grain. Methods. The study analyzed 11 varieties of soft winter wheat, ‘Podolianka’, ‘MIP Yuvileina’, ‘MIP Fortuna’, ‘MIP Roksolana’, ‘MIP Feieriia’, ‘MIP Vidznaka’, ‘MIP Nika’, ‘MIP Darunok’, ‘MIP Aelita’, ‘MIP Aurika’ and ‘MIP Dovira’. The study investigated the impact of preceding crops (soybean, sunflower, corn/MWR, green manure (white mustard), and mustard/seed) and sowing dates (25 September, 5 and 15 October) on grain yield and baking quality. Results. The research was conducted in 2021–2023 on the experimental fields of the V. M. Remeslo Myronivka Institute of Wheat. The weather conditions were characterized by high temperatures and uneven precipitation. The highest winter wheat yields were obtained after green manure as a preceding crop (7.29 t/ha), and the lowest yields after sunflower (5.35 t/ha). Shifting the sowing date from 25 September to 15 October reduced the average yield of the varieties. The only exception to the results were those obtained after crops such as sunflower and mustard when sown on 5th October, which yielded 5.54 and 6.51 t/ha respectively. The highest yields (6.39–6.95 t/ha) were observed for the varieties ‘MIP Darunok’, ‘MIP Aurika’, ‘MIP Aelita’ and ‘MIP Vidznaka’. Higher protein, gluten and sedimentation values were observed after green manure and soya as a preceding crop; in addition, after soya there was a tendency to increase flour strength and bread volume. The aforementioned shift in sowing date also resulted in a decrease in bread volume and an increase in vitreousness; the remaining parameters were within the range of significant differences. The varieties ‘MIP Dovira’, ‘MIP Nika’ and ‘MIP Roksolana’ were characterized by the highest protein content in the grain. According to the complex of quality indicators, ‘MIP Yuvileina’, ‘MIP Roksolana’ and ‘MIP Aurika’ prevailed over others. Conclusions. It was found that green manure and soybean as a preceding crop, as well as optimal sowing dates – the third decade of September and the first of October – provided the maximum yield of the varieties studied. The varieties with the highest yields were ‘MIP Darunok’, ‘MIP Vidznaka’, ‘MIP Aurika’ and ‘MIP Aelita’. According to the complex of grain quality indicators, the varieties ‘MIP Yuvileina’, ‘MIP Roksolana’ and ‘MIP Aurika’ were distinguished.

Yield plasticity of new varieties of soft winter wheat (Triticum aestivum L.) in different soil and climatic conditions of Ukraine

2024-01-22T11:21:36+02:00

Purpose. To determine the level of productivity of new varieties of soft winter wheat, their stability and plasticity when grown in different soil and climatic conditions of Ukraine. Methods. The field trial was carried out in 2022–2023 under the conditions of the research stations of the Ukrainian Institute for Plant Variety Examination (UIPVE) in the Steppe, Forest Steppe and Polissia soilclimatic zones. The peculiarities of plant growth and development, the yield formation of 34 new varieties of soft winter wheat were studied. Plasticity and stability of yield formation were calculated and analyzed according to the Ebergard – Russell method, general homeostaticity and selection value according to Khangildin V.V. and Lytvynenko N.A. In the course of the research, computational and statistical methods were used. Analysis and synthesis were used to draw conclusions. Results. The yield varied from 5.4 to 8.4 t/ha, depending on the soil and climate zone and the location of the trial. The variety ‘LG Optimist’ had the highest average yield (8.1 t/ha) and ‘YUSON’ the lo west (6.0 t/ha). The most favorable conditions for the study of growth and development of genotypes in 2022 were in the Forest-Steppe (Ij = 0.9) and Polissia (Ij = 0.6) zones, in 2023 – Polissia (Ij = 0.2); the worst in 2022 – in the Steppe (Ij = –0.7), in 2023 – in the Steppe (Ij = –0.7) and ForestSteppe (Ij = –0.5) zones. In 2023, the best climatic conditions were in the Polissia zone (Ij = 0.2), the worst were in the points located in the Steppe (Ij = –0.7) and Forest Steppe (Ij = –0.5) zones. The theoretical yield values of the varieties at the research points did not differ from the actual values and averaged 7.1 t/ha, ranging from 6.0 to 8.1 t/ha. High homeostaticity and low level of variation (V ≤ 10.0%) were found in the varieties ‘Yevraziia’ (Hom = 15.6), ‘Dekaster’ (Hom = 15.2), ‘FORSAIT’ (Hom = 13.9) and ‘LG Optimist’ (Hom = 12.1). In addition, ‘LG Optimist’ (Sc = 6.9) and ‘Dekaster’ (Sc = 6.6) had the highest breeding value. The variety ‘FORSAIT’ (bi = 0.2; S2 di = 0.24), with an average yield of 7.4 t/ha, has a high plasticity, so it is recommended to grow it on extensive backgrounds and in unfavorable conditions. Varieties ‘Yevraziia’, ‘Nosivochka’, ‘SOLIFLOR CS’, ‘Mizynka’, ‘Troiana’, ‘LG Optimist’, ‘Biskvit’, ‘MIP Dovira’, ‘Etual’, ‘Tvorchist Odeska’, ‘Attribut’, ‘Epihraf’, ‘Dekaster’, ‘Epitet’, ‘Jannis’, ‘Pamiati Horlacha’, ‘Trembita Bilotserkivska’ and ‘Slava Unavy’ with a combined manifestation of high ecological plasticity bi = 1 and stability S2 di = 0 in average grain yield 7.2 t/ha, according to the genotype respond well to the improvement of growing conditions and are stable, are considered the most practically valuable. Conclusions. New varieties of soft winter wheat, when grown in different soil and climatic conditions, fully develop their genetic potential under significant changes in environmental conditions and produce the highest yield that can be obtained under ideal conditions.

Василь Мединець (1924–2014) – життя, присвячене сортознавству

2024-04-15T15:15:26+03:00

Geneedited plants: achievements and prospects (review)

2024-03-18T11:45:09+02:00

Purpose.To analyze the current state of agricultural crop improvement using gene editing technologies. Results. The current state of plant breeding using gene editing technologies is analyzed. To date, genome editing has been applied to a wide range of crops, including wheat, barley, maize, legumes, soybean, rapeseed, tomato, chicory, various vegetable crops, fruit trees, forest trees and algae. The practical application of these technologies is illustrated by the example of genes associated with ensuring tolerance to high and low temperatures. Examples of commercialized geneedited plants are given. Conclusions. By contributing to increased yields, improved resistance to diseases and pests, and biofortification of food crops, gene editing technology undoubtedly has great prospects and is definitely already the technology for creating improved varieties of agricultural crops.

Compatibility of Actinidia arguta with Schisandra chinensis and Malus domestica plants in mixed plantings

2024-03-18T11:20:50+02:00

The accumulation of photosynthetic pigments, biogenic elements and amino acids in the leaves of A. argute (kiwi berry) and S. chinensis (Chinese magnolia vine) during their cultivation in the same vegetative containers was studied. Different ratios of the number of plants in the containers were used, namely 50%:50%, 33%:67%, and 67%:33%. Single-species plantings were used as a control. Research demonstrated that the ratios between co-planted plants can impact the levels of photosynthetic pigments, biogenic elements, and amino acids in their leaves. Mixed planting was found to decrease the concentration of photosynthetic pigments in A. argute leaves under these conditions. The chlorophyll a content showed significant changes, decreasing by 6.7–18.7% with S. chinensis ratio in the container and by 31.3–33.8% with M. domestica ratio compared to mono-planting. Chlorophyll b also showed differences, ranging from 1.2–8.6% and 9.7–29.7%, respectively. Additionally, certain features were observed in the distribution of mineral nutrition elements in plant tissues of A. arguta. In mono-planting conditions, the leaves of plants showed an increase in magnesium, potassium, and phosphorus content. When actinidia is grown together with Chinese magnolia vine and an apple tree, especially with an apple tree, the supply of nitrogen, phosphorus, potassium, and calcium to the plants is sharply reduced. By the end of the growing season of plants, the total content of free amino acids in A. arguta leaves increased in mono-planting conditions. Actinidia plants exhibited a disturbance in phosphate metabolism in mixed plantings, as evidenced by elevated levels of arginine and histidine, as well as altered nitrogen metabolism indicated by decreased concentrations of glutamic acid in the leaves. The competitiveness analysis, using the Vanderbeng and Ennik method, showed that A. arguta plants are more compatible with S. chinensis than with M. domestica. The productivity and sustainability of crops can be significantly increased by using the method of mixed crops in agrophytocenoses. However, it is necessary to consider the bioecological features of plants and their tolerance to root secretions of other species. A. arguta and S. chinensis are forest lianas that are a unique part of forest ecosystems. They grow in multispecies groups in natural conditions, so it can be expected that they will show tolerance in mixed plantings.