Analysis of electrophoretic spectra of zeins for the evaluation of the genetic diversity of maize lines (<i>Zea mays</i> (L.) Merr.)

Authors

DOI:

https://doi.org/10.21498/2518-1017.14.1.2018.126517

Keywords:

polymorphism of reserve proteins, PAGE electrophoresis, genetic distances, relative electrophoretic mobility, maize, zein

Abstract

Purpose. To evaluate zein electrophoretic spectra of maize lines in order to create the panel for certification.

Methods. Electrophoresis of reserve proteins, cluster analysis.

Results. Findings of investigations of maize line 31 on reserve proteins in seeds using electrophoretic separation of zein components are presented. Based on the determination of the relative electrophoretic mobility of zeins, it was found that the studied maize lines are homogeneous and have differences in zeins component composition, which allows to identify them. It was defined that the most similar lines were ‘ДК744’ and ‘ДК298’, ‘ДК3070’ and ‘ДК633/325’, ‘PLS61’ and ‘Chi31’ in which more spectra with the same relative electrophoretic mobility were revealed. The unique components of zein 96, 32 and 41 are determined in the studied lines ‘ДК267’, ‘ДК129-4’ and ‘ДК236’. Cluster analy­sis allowed to define that the lines ‘ДК315’, ‘ДК267’ and ‘ДК276’ were at the greatest distance from all the studied lines. According to genetic distances, a sufficient level of similarity (300–400) for zein spectra is noted between lines of Iodent and Lancaster germplasms.

Conclusions. For eva­luation of maize gene pool, it is advisable to use the method of electrophoresis of reserve proteins, because it allows to evaluate their homogeneity and define the level of genetic kinship. It is defined that the most distant lines among the studied ones are ‘ДК315’, ‘ДК267’ and ‘ДК276’. It has been established that the well-known reference lines ‘PLS61’ and ‘Chi31’ formed a separate cluster. Thus, the use of the method of electrophoresis of reserve proteins allowed to define the most distant lines with common type of germplasm and identify similar components of zein in lines of different genoplasm.

Author Biographies

Л. М. Присяжнюк, Ukrainian Institute for Plant Variety Examination

Prysiazhniuk, L. M.

Т. М. Сатарова, Ukrainian Institute for Plant Variety Examination

Satarova, T. M.

С. О. Ткачик, Ukrainian Institute for Plant Variety Examination

Tkachyk, S. O.

Ю. В. Шитікова, Ukrainian Institute for Plant Variety Examination

Shytikova, Yu. V.

Б. В. Дзюбецький, Institute of Grain Crops, NAAS of Ukraine

Dzyubetskyi, B. V.

В. Ю. Черчель, Institute of Grain Crops, NAAS of Ukraine

Cherchel, V. Yu.

References

Zhang, Z., Yang, J., & Wu, Y. (2015). Transcriptional regulation of zein gene expression in maize through the additive and syner­gistic action of opaque2, Prolamine-box binding factor, and O2 heterodimerizing proteins. Plant Cell., 27(4), 1162–1172. doi: 10.1105/tpc.15.0003.

Sidorova, V. V., Konarev, A. V., Kerv, Yu. A., & Matveeva, G. V. (2015). Protein Markers in the Analysis of a Genetic Variety, Breeding, and the Corn Seed Control. Agrarnaya Rossiya [Agrarian Russia], 12, 2–10. [in Russian]

Tyutyunov, S. I., Voronin, A. N., Zhurba, G. M., & Khoroshilov, S. A. (2017). Main results of maize hybrids breeding at Belgorod Research Institute of Agriculture. In Selektsiya rasteniy: proshloe, nastoyashchee i budushchee: sbornik materialov I Vseros. nauchno-prakt. konf. s mezhdunar. uchastiem [Breeding of plants: past, present and future: a collection of materials of the 1st All-Russian Scientific and Practical Conference with international participation] (pp. 180–185). Nov. 24–26, 2016, Belgorod, Russia. [in Russian]

Iqbal, J., Shinwari, Z. K., Rabbani, M. A., & Khan, S. (2014). Genetic variability assessment of maize (Zea mays L.) germplasm based on total seed storage proteins banding pattern using SDS-PAGE. Eur. Acad. Res., 2(2), 2144–2160.

Zayakina, G. V., & Sozinov, A. A. (1993). Genetic analysis of maize prolamins (zeins). TSitol. Genet. [Cytology and Genetics], 27(5), 23–26. [in Russian]

Sidorova, V. V., Matveeva, G. V., Kerv, Yu. A., & Konarev, A. V. (2012). Possibilities of zein markers use to increase efficiency of maize heterosis breeding. Trudy po prikladnoy botanike, genetike i selektsii [Proceedings on Applied Botany, Genetics and Breeding], 170, 147–157. [in Russian]

Wall, J. S., Fey, D. A., & Paulis, J. W. (1984). Improved two-dimensional electrophoretic separation of separation to study of zein inheritance in corn genotypes. Cereal chem, 61(2), 141–146.

Yao, D., Qi, W., Li, X., Yang, Q., Yan, S., Ling, H., … Song, R. (2016). Maize opaque10 encodes a cereal-specific protein that is essential for the proper distribution of zeins in endosperm protein bodies. PLoS Genet., 12(8), e1006270. doi: 10.1371/journal.pgen.1006270.

Zayakina, G. V., & Sozinov, A. A. (1997). Genetic analysis of maize zeins: inheritance of minor components. TSitol. Genet. [Cytology and Genetics], 31(4), 5–11. [in Russian]

Bielikov, Ye. I., & Kuprichenkova, T. H. (2015). Study of yielding capacity of early-season hybrids of various heterotic models under the conditions of the Steppe zone of Ukraine. Biuleten Instytutu silskoho hospodarstva stepovoi zony NAAN Ukrainy [Bulletin of the Institute of Agriculture of the Steppe zone, NAAS of Ukraine], 9, 58–62. [in Ukrainian]

Tkachyk, S. O. (Ed.). (2015). Metodyka provedennia kvalifikatsiinoi ekspertyzy sortiv roslyn na prydatnist do poshyrennia v Ukraini. Metody vyznachennia pokaznykiv yakosti produktsii roslynnytstva [Regulations on the procedure and the conduct of qualification tests for suitability of crop varieties for dissemination in Ukraine. Methods of determining quality indices of crop products]. Vinnytsia: Nilan-LTD. [in Ukrainian]

Zayakina, G. V., Popov, V. N., & Sozinov, A. A. (1998). Localization of zein coding clusters on chromosome 4 in maize. Genetika [Genetics], 34(7), 1000–1003. [in Russian]

Sidorova, V. V., Konarev, A. V., Matveeva, G. V., & Timofeeva, G. I. (2014). The use of zein electrophoretic spectrum to predict hete­rosis in corn. Agrarnaya Rossiya [Agrarian Russia], 6, 34–41. [in Russian]

Sidorova, V. V., Matveeva, G. V., Konarev, A. V., Kerv, Yu. A., Kud­ryavtsev, A. M., Upelniek, V. P., & Yankovskiy, N. K. (2012). Zein Markers in Maize Genepool Analysis and Breeding Improvement. Agrarnaya Rossiya [Agrarian Russia], 7, 5–11. [in Russian]

Ermantraut, E. R., Prysiazhniuk, O. I., & Shevchenko, I. L. (2007). Statystychnyi analiz ahronomichnykh doslidnykh danykh v paketi STATISTICA 6.0 [Statistical analysis of agronomic study data in the software suite Statistica 6.0]. Kyiv: PolihrafKonsaltynh. [in Ukrainian]

Everitt, B. S., Landau, S., Leese, M., & Stahl, D. (2011). Cluster Analy­sis. (5th ed.). Chichester: Wiley. doi: 10.1002/9780470977811

Bänziger, M., Setimela, P. S., Hodson, D., & Vivek, B. (2004). Bree­ding for improved drought tolerance in maize adapted to southern Africa. In New directions for a diverse planet: Proc. 4th Int. Crop Sci. Congress. Sept. 26 – Oct. 1, 2004, Brisbane, Australia. Retrieved from www.cropscience.org.au/icsc2004/pdf/152_banzigerm.pdf

Sidorova, V. V., Kerv, Yu. A., Matveeva, G. V., & Konarev, A. V. (2014). Identification of the genetic diversity of corn for protein spectra in the solution of fundamental and applied tasks of resources and breeding of crop. In Geneticheskie resursy rasteniy – osnova prodovol’stvennoy bezopasnosti i povysheniya kachestva zhizni: tezisy dokladov Mezhdunar. nauch. konf. [Genetic resources of plants are the basis of food security and improving the quality of life: Abst. Int. Sci. Conf.] (p. 90). Oct. 68, 2014, Saint Petersburg, Russia.

Komarova, G., Rotar, A., Paliy, A., & Mikhalaki, A. (2017). The expression of the effect of heterosis in corn at the level of protein mar­kers. ?Ştiina agricol?ă [Agricultural Science], 2, 1115. [in Russian]

Satarova, T. M., Borysova, V. V., Cherchel, V. Yu., Derkach, K. V., & Abraimova, O. Ye. (2013). Genetic distances of corn lines and their cytosterile analogues for polymorphism of SNP markers. Selektsia i Nasinnitstvo [Plant Breeding and Seed Production], 103, 124134. [in Ukrainian]

Satarova, T. M., Derkach, K. V., & Abraimova O. Ye. (2011). Evaluation of the reciprocal effect in vitro culture in genotypes of corn germ plasma Lancaster. Biuleten Instytutu zernovoho hospodarstva [Bulletin of the Institute of Grain Farming], 40, 2024. [in Ukrainian]

El’konin, L. A., Domanina, I. V., & Ital’yanskaya, Yu. V. (2016). Genetic engineering as a tool for modifying the composition of reserve proteins and improving the nutritional value of grain in cereals. Sel’skokhozyaistvennaya biologiya [Agricultural Вiology], 51(1), 1730. doi: 10.15389/agrobiology.2016.1.17rus. [in Russian]

Zheng, X.-Q., Liu, X.-L., Yu, S.-F., Wang, X.-J., Ma, Y.-Q., Yang, S., & Jing, S.-S. (2014). Effects of extrusion and starch removal pretreatment on zein proteins extracted from corn gluten meal. Cereal Chem., 91(5), 496–501. doi: 10.1094/CCHEM-07-13-0141-R.

Liu, H., Shi, J., Sun, C., Gong, H., Fan, X., Qiu, F., … Wu, Y. (2016). Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize. Proc. Natl. Acad. Sci., 113(18), 4964–4969. doi: 10.1073/pnas.1601352113

Published

2018-03-21

How to Cite

Присяжнюк, Л. М., Сатарова, Т. М., Ткачик, С. О., Шитікова, Ю. В., Дзюбецький, Б. В., & Черчель, В. Ю. (2018). Analysis of electrophoretic spectra of zeins for the evaluation of the genetic diversity of maize lines (&lt;i&gt;Zea mays&lt;/i&gt; (L.) Merr.). Plant Varieties Studying and Protection, 14(1), 89–96. https://doi.org/10.21498/2518-1017.14.1.2018.126517

Issue

Section

GENETICS

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