Variability of morphometric traits of seeds of different genotypes of Lycium spp.




goji berry, cultivars, varieties, seeds, parameters, cluster hierarchical analysis


Purpose. The objective of this study was to evaluate the morphological parameters of Lycium spp. seeds from the collections in M. M. Hryshko National Botanical Garden (NBS) NAS of Ukraine.

Methods. Cultivars and varieties of three Lycium species (Lycium barbarum, L. chinense, L. truncatum) were studied in the period from 2016 till 2019. The following morphometric measurments were conducted: seeds weight, seeds length, seeds width and index of seeds shape. Basic statistical analyses were performed using PAST 2.17. Hierarchical cluster analyses of similarity between genotypes were computed on the basis of the Bray-Curtis similarity index. Correlation between traits was determined using the Pearson correlation coefficient.

Results. Cultivars and varieties of different species of Lycium varied in weight, shape, and size of seeds. Seed weight varied from 0.54 to 3.54 mg, seed length from 1.90 to 3.06 mm, seed width from 1.43 to 2.53 mm. The shape indexes of seeds were found ranging from 0.73 to 0.80. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium spp. cultivars and varieties. The most variable features: seeds weight (8.51–28.22%) and seeds length (5.07–24.81%) are important parameters for selection. The use of cluster analysis made it possible to establish the similarity between the species of the studied Lycium species.

Conclusions. Diagnostic signs by seed morphometry for differentiation of Lycium species were revealed. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium cultivars and varieties. The most variable characteristics of the studied genotypes were seed weight and length, which are important parameters for selection because they determine the pulp content and number of seeds, as well as the ratio of these parameters between them. It is through variability that promising varieties with low seed weight and length can be selected, Due to securing them later vegetatively.


Ivanišová, E., Grygorieva, O., Abrahamová, V., Schubertova, Z., Terentjeva, M., & Brindza, J. (2017). Characterization of morphological parameters and biological activity of jujube fruit (Ziziphus jujuba Mill.). J. Berry Res., 7, 249–260. doi: 10.3233/JBR-170162

Vinogradova, Yu., Grygorieva, O., Vergun, O., & Brindza, J. (2017). Morphological characteristics for fruits of Aronia mitschurinii A.K.Skvortsov & Maitul. Potr. S. J. F. Sci., 11(1), 754–760. doi: 10.5219/845

Grygorieva, O., Klymenko, S., Ilinska, A., & Brindza, J. (2018). Variation of fruits morphometric parameters of Elaeagnus multiflora Thunb. germplasm collection. Potr. S. J. F. Sci., 12(1), 527–532. doi: 10.5219/922

Grygorieva, O., Klymenko, S., Vinogradova, Y., Motyleva, S., Gur­nenko, I., Piórecki, N., & Brindza, J. (2018). Study of morphological characteristics of pollen grains of Aronia Mitschurinii A.K.Skvortsov & Maitul. Agrobiodivers. Improv. Nutr., Health Life Qual., 2, 49–56. doi: 10.15414/agrobiodiversity.2018.2585-8246.049-056

Grygorieva, O., Klymenko, S., Vinogradova, Y., Vergun, O., & Brindza, J. (2018). Variation in morphometric traits of fruits of Mespilus germanica L. Potr. S. J. F. Sci., 12(1), 782–788. doi: 10.5219/999

Horčinová Sedláčková, V., Grygorieva, O., Vergun, O. M., Vinogradova, Ju. K., & Brindza, J. (2019). Comparison of selected characteristics of cultivars and wild-growing genotypes of Sambucus nigra in Slovakia. Biosyst. Divers., 27, 56–61. doi: 10.15421/011909

Barthlott, W., & Ziegler, B. (1981). Seed coat morphology as a systematic characteristic in orchids. Ber. Deutsch. Bot. Ges., 94, 267–273. doi: 10.1111/j.1438-8677.1981.tb03402.x

Rani, U., Singh, S. G., Gupta, S., & Garg, V. (1993). Morphometry of orchid seeds in Epidendroidae as revealed by SEM. Adv. Plant Sci., 6, 128–133.

Augustine, J., Yogendra, K., & Sharma, J. (2001). Orchids of India-II. Biodiversity and status of Bulbophyllum Thou Daya publishing house. New Delhi: Trinagar.

Levin, R. A., Bernardello, G., Whiting, C., & Miller, J. S. (2011). A new generic circumscription in tribe Lycieae (Solanaceae). Taxon, 60(3), 681–690. doi: 10.1002/tax.603005

Barboza, G. E., Hunziker, A. T., Bernardello, G., Cocucci, A. A., Carrizo Garcia, C., … Anton, A. (2016). Solanaceae. In J. W. Kadereit and V. Bittrich (Eds.), The Families and Genera of Vascular Plants (Vol. 14, pp. 295–357). New Delhi: Springer. doi: 10.1007/978-3-319-28534-4

Yao, R., Heinrich, M., & Weckerle, C. S. (2018). The genus Lycium as food and medicine: A botanical, ethnobotanical and historical review. J. Ethnopharmacol., 212, 50–66. doi: 10.1016/j.jep.2017.10.010

Amagase, H., & Farnsworth, N. R. (2011). A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji). Food Res. Int., 44(7), 1702–1717. doi: 10.1016/j.foodres.2011.03.027

Wang, C. C., Chang, S. C., Inbaraj, B. S., & Chen, B. H. (2010). Isolation of carotenoids, flavonoids and polysaccharides from Lycium barbarum L. and evaluation of antioxidant activity. Food Chem., 120(1), 184–192. doi: 10.1016/j.foodchem.2009.10.005

Chang, J., Zhou, Z.-W., Sheng, H.-P., He, L.-J., Fan, X.-W., He, Z.-X., & Zhou, S.-F. (2015). An evidence-based update on the pharmacological activities and possible molecular targets of Lycium barbarum polysaccharides. Drug Des. Dev. Ther., 9, 33–78. doi: 10.2147/DDDT.S72892

Niro, S., Fratianni, A., Panfili, G., Falasca, L., Cinquanta, L., & Alam, M. R. (2017). Nutritional evaluation of fresh and dried goji berries cultivated in Italy. Ital. J. Food Saf., 29(3), 398–408. doi: 10.14674/1120-1770/ijfs.v649

Qian, D., Yang, J., Kang, L., Ji, R., & Huang, L. (2017). Variation of sweet chemicals in different ripening stages of wolfberry fruits. Chin. Herb. Med., 9(4), 329–334. doi: 10.1016/s1674-6384(17)60112-6

Potterat, O. (2010). Goji (Lycium barbarum and L. chinense): Phytochemistry, pharmacology and safety in the perspective of traditional uses and recent popularity. Planta Med., 76(1), 7–19. doi: 10.1055/s-0029-1186218

Protti, M., Gualandi, I., Mandrioli, R., Zappoli, S., Tonelli, D., & Mercolini, L. (2017). Analytical profiling of selected anti­oxidants and total antioxidant capacity of goji (Lycium spp.) berries. J. Pharm. Biomed. Anal., 143, 252–260. doi: 10.1016/j.jpba.2017.05.048

Wang, S., Suh, J. H., Zheng, X., Wang, Y., & Ho, C. T. (2017). Identification and quantification of potential anti-inflam­matory hydroxycinnamic acid amides from wolfberry. J. Agric. Food Chem., 65(2), 364–372. doi: 10.1021/acs.jafc.6b05136

Cumaoglu, A., Bekci, H., Ozturk, E., Yerer, M. B., Baldemir, A., & Bishayee, A. (2018). Goji berry fruit extracts suppress proliferation of triple-negative breast cancer cells by inhibiting EGFR-Mediated ERK/MAPK and PI3K/Akt signaling pathways. Nat. Prod. Commun., 13(6), 701–706. doi: 10.1177/1934578x1801300613

Wojdyło, A., Nowicka, P., & Babelewski, P. (2018). Phenolic and carotenoid profile of new goji cultivars and their anti-hyperglycemic, anti-aging and antioxidant properties. J. Funct. Foods, 48, 632–642. doi: 10.1016/j.jff.2018.07.061

Ma, Z. F., Zhang, H., Teh, S. S., Wang, C. W., Zhang, Y., Hayford, F., ... Zhu, Y. (2019). Goji berries as a potential natural antioxidant medicine: An insight into their molecular mechanisms of action. Oxid. Med. Cell. Longev., 2019, 2437397. doi: 10.1155/2019/2437397

Chen, P.-Y., Shih, T.-H., Chang, K.-C., Wang, J.-S., Yang, C.-M., & Chang, Y.-S. (2020). Potential of galled leaves of Goji (Lycium chinense) as functional food. BMC Nutr., 6, 26. doi: 10.1186/s40795-020-00351-w

Grygorieva, O., Vergun, O., Klymenko, S., Zhurba, M., Horčinová Sedláčková, V., Ivanišová, E., & Brindza, J. (2020). Estimation of phenolic compounds content and antioxidant activity of leaves extracts of some selected non-traditional plants. Potravinarstvo Slovak Journal of Food Sciences, 14, 501–509. doi: 10.5219/1314

Szot, I., Zhurba, M., & Klymenko, S. (2020). Pro-health and functional properties of goji berry (Lycium spp.). Agrobiodivers. Improv. Nutr., Health Life Qual., 4, 134–145. doi: 10.15414/agrobiodiversity.2020.2585-8246.134-145

Kazbekovna, S. F., Sekinaeva, M. A., & Denisenko, O. N. (2018). Comparative micromorphological investigations of red godji berries (Lycium barbarum L.) and black godji berries (Lyci­um ruthenicum Murr.). Pharmacogn. J., 10(5), 911–915. doi: 10.5530/pj.2018.5.153

Zhang, Z. Y., Lu, A. M., & D’Arcy, W. G. (1994). Solanaceae. In Z. Y. Wu, & P. H. Raven (Eds.), Flora of China (Vol. 17, pp. 330–332). Beijing: Science Press; Saint Louis: Missouri Botanical Garden Press.

Yao, R., Heinrich, M., & Weckerle, C. (2018). The genus Lycium as food and medicine: a botanical, ethnobotanical and historical review. J. Ethnopharmacol., 212, 50–66. doi: 10.1016/j.jep.2017.10.010

Jaćimović, V., Božović, D., Ercisli, S., Ognjanov, V., & Bosančić, B. (2015). Some Fruit Characteristics of selected cornelian cherries (Cornus mas L.) from Montenegro. Erwerbs-Obstbau, 57, 119–124. doi: 10.1007/s10341-015-0238-6

Ruzdik, N. M., Karov, I., Mitrev, S., Gorgieva, B., Kovacevik, B., & Kostadinovska, E. (2015). Evaluation of sunflower (Helianthus annuus L.) varieties using multivariate statistical analysis. Helia, 38(63), 1–12. doi: 10.1515/helia-2015-0007

Jiang, Y. D., Cao, J., Dong, Q. Z., & Wang, S. R. (2007). Expe­rimental study of anti-atherosclerosis potency by lycium seed oil and its possible mechanism. Zhong Yao Cai, 30(6), 672–677.

Li, G., You, J., Suo, Y., Song, C., Sun, Z., Xia, L., … Shi, J. (2011). A developed pre-column derivatization method for the determination of free fatty acids in edible oils by reversed-phase HPLC with fluorescence detection and its application to Lycium barbarum seed oil. Food Chem., 125, 1365–1372. doi: 10.1016/j.foodchem.2010.10.007

Liu, Z., Liu, B., Kang, H., Yue, H., Chen, C., Jiang, L., & Shao, Y. (2019). Subcritical fluid extraction of Lycium ruthenicum seeds oil and its antioxidant activity. J. Food Sci. Technol., 54(1), 161–169. doi: 10.1111/ijfs.13920




How to Cite

Zhurba, M. Y., Klymenko, S. V., & Szot, I. (2021). Variability of morphometric traits of seeds of different genotypes of Lycium spp. Plant Varieties Studying and Protection, 17(1), 5–13.