Agrobiological evaluation of collection of vegetable soybean varieties in the Forest-Steppe of Ukraine




edamame, yield, protein, sugar content, seeds


Purpose. Agrobiological assessment of soybean varieties Glycine max var. Shirofumi on a complex of economically valuable traits for introduction in the conditions of the Forest-Steppe of Ukraine. Selection of promising breeding forms based on morpho-biological and physiological-biochemical characteristics.

Methods. Field, laboratory, statistical, computatio­nal and analytical. The studies were carried out in the conditions of the educational and production department of Uman National University of Horticulture during 2020–2021, using collection varieties of different ecological and geographical origin (Ukraine, Belarus, Sweden, Japan and Russia). The cultivars were assessed according to the following parameters: plant height, leaf area, net productivity of photosynthesis and indices of individual productivity (weight of beans per plant, number of seeds in a bean, etc.), productivity of green beans and biologically mature seeds and, accordingly, quality indicators of production (dry matter, sugar and protein content). The counts were carried out in the phase of the technical ripeness of the beans.

Results. The variability of the “plant height” trait of the studied varieties had an average variation – the coefficient of variation was 22%. The results showed that the standard cultivar ‘Romatnyka’ and the collection cultivars ‘Karikachi’ and ‘Astra’ belong to the semi-determinant type of growth (97–109 cm), cultivar ‘Fiskeby V’, ‘L 380-2-13’, ‘Fiskeby V-E5’, ‘SibNIISOKh 6’, ‘Sac’, ‘Vesta’ belong to the determinant type of growth. According to the number of seeds in the pod, the studied varieties were clearly divided into two groups: with two-seeded beans (varieties ‘Karikachi’, ‘Astra’, ‘L 380-2-13’) and three-seeded beans [varieties ‘Romatnyka’ (standard), ‘Fiskeby V’, ‘Vesta’, ‘SibNIISOX 6’, ‘Sac’, ‘Fiskeby V-E5’]. The maximum yield of edamame beans was produced by varieties ‘L 380-2-13’ (17.3 t/ha), ‘Vesta’ (18.8 t/ha), ‘Sac’ (19.6 t/ha), ‘Fiskeby V’ (21.4 t/ha), ‘Fiskeby V-E5’ (22.4 t/ha). A significant differentiation of soybean varieties in the biochemical composition of immature beans was revealed. The dry matter content was 22.70–31.70%. The share of protein in edamame green beans was 28.2–38.6%, in biologically mature seeds its share increased to 36.1–42.8%. Among soluble sugars, the highest concentration was noted for sucrose – 7.70–9.38 mg/100 g in dry seeds, what in average amounted to 81.6–86.2% of all sugars. The presented results provide a comprehensive assessment of breeding work on soybean varieties with a low content of oligosaccharides.

Conclusions. Evaluation of collection varieties of vegetable soybeans by the variability of morphological traits and productivity made it possible to distinguish ‘Sac’ variety by a complex of valuable traits for creation of new varieties of vegetable soybeans adapted to the conditions of the Forest-Steppe of Ukraine.


Johnson, D., Wang, S., & Suzuki, A. (1999). Edamame: a vegetab­le soybean for Colorado. In J. Janick (Ed.), Perspectives on New Crops and New Uses (pp. 385–387). Alexandria, VA: ASHS Press.

Mentreddy, S. R., Mohamed, A. I., Joshee, N., & Yadav, A. K. (2002). In Edamame: a nutritious vegetable crop. Trends in new crops and new uses (pp. 432–438). Atlanta, GA: ASHS Press.

Zeipinё А. S., Alsinё А. I., & Lepse, L. (2017). Insight in edamame yield and quality parameters: A review. Res. Rural. Dev., 2, 40–44. doi: 10.22616/rrd.23.2017.047

Yu, D., Lord, N., Polk, J., Dhakal, K., Li, S., Yin, Y., … Huang, H. (2021). Physical and chemical properties of edamame during bean development and application of spectroscopy-based machine learning methods to predict optimal harvest time. Food Chem., 368, 130799. doi: 10.1016/j.foodchem.2021.130799

Carneiro, R., Duncan, S., O’Keefe, S., Yu, D., Huang, H., Yin, Y., … Gillen, A. (2021). Utilizing Consumer Perception of Edamame to Guide New Variety Development. Front. Sustain. Food Syst., 4, 556–580. doi: 10.3389/fsufs.2020.556580

Hymowitz, T. (1984). Dorsett-Morse soybean collection trip to East Asia: 50-year retrospective. Econ. Bot., 38(4), 378–388. doi: 10.1007/BF02859075

Smith, J. M., & Van Duyne F. O. (1951). Other soybean products. In K. S. Markley (Ed.), Soybeans and soy-bean products (Vol. 2, pp. 1055–1078). New York, NY: Interscience.

Carneiro, R. C. V., Duncan, S. E., O’Keefe, S. F., Yin, Y., Neill, C. L., & Zhang, B. (2020). Sensory and consumer studies in plant breeding: A guidance for edamame development in the U.S. Front. Sustain. Food Syst., 4, 124. doi: 10.3389/fsufs.2020.00124

Zhang, B., Lord, N., Kuhar, T., Duncan, S., Huang, H., Ross, J., … Buss, G. (2021). A vegetable soybean cultivar for commercial edamame production in the mid-Atlantic USA. J. Plant Regist., 1–5. doi: 10.1002/plr2.20140

Williams, M. M. (2015). Phenomorphological characterization of vegetable soybean germplasm lines for commercial production. Crop Sci., 55, 1274–1279. doi: 10.2135/cropsci2014.10.0690

Nolen, S., Zhang, B., & Kering, M. K. (2016). Increasing fresh edamame bean supply through season extension techniques. J. Hortic., 3, 1–5. doi: 10.4172/2376-0354.1000170

Xu, Y., Cartier, A., Kibet, D., Jordan, K., Ivy, H., & Davis, S. (2016). Physical and nutritional properties of edamame seeds as influenced by stage of development. J. Food Meas. Cha­ract., 10, 193–200. doi: 10.1007/s11694-015-9293-9

Jankauskienė, J., Brazaitytė, A., & Vaštakaitė-Kairienė, V. (2021). Potential of vegetable soybean cultivation in Lithuania. Not. Bot. Horti Agrobot. Cluj-Napoca, 49, 12267. doi: 10.15835/nbha49112267

Findlay, S., McKenzie, J., Al-Dujaili, E., & Davidson, H. (2015). Weight loss and reductions in body mass index, abdominal-girth and depth after a 12 week dietary intervention of soya beans (edamame). Proc. Nutr. Soc., 74, E70. doi: 10.1017/S0029665115000853

Nedvyha, M. V. (1994). Morfolohichni kryterii ta henezys sucha­snykh gruntiv Ukrainy [Morphological criteria and genesis of modern soils of Ukraine]. Kyiv: Ahrarna nauka. [in Ukrainian]

Produkty pererobliannia fruktiv ta ovochiv. Metody vyznachannia sukhykh rechovyn abo volohy: DSTU 7804:2015 [Fruit and vege­table products. Methods for determination of total solids or moisture: State standard of Ukraine 7804:2015]. (2015). Kyiv: Derzhspozhyvstandart Ukrainy. [in Ukrainian]

Johansen, H. N., Glitso, V., & Knudsen, K. E. B. (1996). Influence of extraction solvent and temperature on the quantitative determination of oligosaccharides from plant materials by high-performance liquid chromatography. J. Agric. Food Chem., 44, 1470–1474. doi: 10.1021/jf950482b

Kormy dlia tvaryn. Vyznachannia vmistu azotu i obchysliuvannia vmistu syroho bilka. Metod Kieldalia: DSTU ISO 5983-2003 [Animal feed. Determination of nitrogen content and calculation of crude protein content. Kjeldahl method: State standard of Ukraine ISO 5983-2003]. Kyiv: Derzhspozhyvstandart Ukrainy. [in Ukrainian]

Lakin, G. F. (1990). Biometriya [Biometrics]. (4th ed., rev. аnd enl.). Moscow: Vysshaya shkola. [in Russian]

Pylnev, V. V. (Ed.). (2005). Chastnaya selektsiya polevykh kul’tur [Private breeding of field crops]. Moscow: Kolos. [in Russian]

Carneiro, R., Yin, Y., Duncan, S., & O’Keefe, S. (2021). Edamame Flavor Characteristics Driving Consumer Acceptability in the United States: A Review. ACS Food Sci. Technol., 1, 1748–1756. doi: 10.1021/acsfoodscitech.1c00261



How to Cite

Yatsenko, V., Poltoretskyi, S., & Yatsenko, A. (2021). Agrobiological evaluation of collection of vegetable soybean varieties in the Forest-Steppe of Ukraine. Plant Varieties Studying and Protection, 17(4), 327–334.