Peculiaritis of Apomictic plants development Pyrus communis х Chaenomeles japonica (Thunb.) Lindl. in vitro conditions
DOI:
https://doi.org/10.21498/2518-1017.2(19).2013.58547Keywords:
apomictic plants, micropropagation, roots, somatic embryogenesisAbstract
The development features of four samples of apomictic plants during micropropagation are presented. These plants have been derived from embryos after pear pollination with the pollen of Chaenomeles japanica at 55 and 70 days of their development. Different responses of samples on the tested concentration of cytokinin (6BAP) and its combination with gibberell acid are shown. Tendency of increasing of the coefficient of the reproduction of separate numbers of apomictic plants has been noted under changing of BAP concentration from 1 mg/l to 2 mg/l or BAP combination with GA3. For the purpose of optimization of the stage of conglomeration forming (buds and shoots) BAP concentration 1 and 2 mg/l should be alternated in a passage on the background of GA3 1,5 mg/l. For the first time the origin of apomictic plant roots during rhizogenesis has been retraced and the anatomical structure of roots in conditions in vitro has been studied. Root formation in vitro occurs in internal tissues of a shoot. Roots of apomictic plants formed in vitro are primary as in plants developed in vivo.Downloads
References
Долматов, Е.А. Стимулятивный апомиксис и проблема получения гомозиготных растений у груши / Е.А. Долматов // Селекция и сорторазведение садовых культур. – Орел: ВНИИСПК, 1996. – С. 111– 122.
Долматов, Е.А. Апомиксис и экспериментальная гаплоидия у груши / Е.А. Долматов, Н.И. Панова // Селекция и сорторазведение садовых культур. – Орел: ВНИИСПК, 1998. – С. 276–280.
Юдин, Б.Ф. Экспериментальная полиплоидия и изучение апомиксиса у кукурузы. / Б.Ф. Юдин // Апомиксис и селекция. – Москва: Наука, 1970. – С. 66–72.
Арендт, Н.К. Изменчивость апомиктических сеянцев некоторых видов фикуса / Н.К. Арендт // Апомиксис и селекция. – Москва: Наука, 1970. – С. 55–66.
Здруйковская-Рихтер, А.И. Культивирование апомиктических зародышей in vitro / А.И. Здруйковская-Рихтер // Апомиксис и селекция. – Москва: Наука, 1970. – С. 165–170.
Джафарова, В.Е. Элементы технологического процесса получения апомиктических растений груши с использованием методов in vitro / В.Е. Джафарова, Е.А. Долматов, Л.В. Ташматова // Методические рекомендации. – Орел, 2011. – 20 с.
Высоцкий, В.А. Использование микропрививок при клональном микроразмножении косточковых культур / В.А. Высоцкий, Е.В. Олешко / Сельскохозяйственная биология. – 1988. – No 4. – С. 75–77.
Майорова, Ю.А. Оптимизация этапов клонального микроразмножения гибридов вишни на основе применения новых биологически активных веществ / Ю.А. Майорова: автореферат дис. на соиск. учен. степени канд. с.-х. наук. – Краснодар, 2009. – 24 с.
Матушкина, О.В. Влияние некоторых биологических, морфологических и механических факторов на регенерацию яблони и груши in vitro / О.В. Матушкина, И.Н. Пронина // Плодоводство и ягодоводство России. – Москва, 2011. – Т. XXVI. – С. 63–69.
Паушева, З.П. Практикум по цитологии растений / З.П. Паушева. – Москва: ВО «Агропромиздат», 1988. – 271 с.
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