Clonal micropropagation of peppermint (Mentha piperita L.) varieties of Ukrainian breeding
Keywords:Mentha piperita L., explant, sterilization, culture in vitro, growth regulators, micropropagation, rhizogenesis, adaptation
Purpose. Developing technology for clonal micropropagation of peppermint (Mentha piperita L.) plants of Ukrainian breeding based on the complex of methods of isolated tissue and organ culture in vitro. Methods. During the experiment, such methods as isolated tissue and organ culture in vitro, clonal micropropagation, detached scion grafting, chemotherapy with adding of virucide Ribavirin to the nutrient medium, biometric and statistical ones were used.
Results. The stepped procedure of sterilization that we have developed allows to receive 88–100% of sterile explants. For M. piperita L. introduction into culture and clonal micropropagation, Murashige and Skoog (MS) nutrient medium appeared to be optimal supplemented with 6-benzylaminopurine (0.75 mg/l), adenine (0.05 mg/l), indole-3-acetic acid (IAA) (0.05 mg/l) and gibberellic acid (0.5 mg/l) on which the reproduction ratio on the 28th day ranged between 1:7 and 1:15. For recovery of plants from viral infection, virucide Ribavirin at concentration of 10 mg/l was added to the nutrient medium. The proposed nutrient medium for rhizogenesis, that contained IAA (0.5 mg/l) and indole butyric acid (IBA) (0.5 mg/l), allows to obtain the frequency of rhizogenesis up to 84–100%. Regenerated plants were adapted to the conditions in vivo on substrate peat : universal soil : perlite : sand in the ratio 2:1:1:1. The survival rate for peppermint varieties amounted to 96–100%. Conclusions. Biotechnological scheme was developed that permits to get healthy, purebred planting material and intensively propagate plants for supplying breeding programs of the Experimental Station for Medicinal Plants of the Institute of Agroecology and Environmental Management of the National Academy of Agrarian Sciences of Ukraine, among which such varieties as ‘Lebedyna pisnia’ and ‘Ukrainska pertseva’ were selected as the most promising for clonal micropropagation.
Andrianov, K. V., Fedchenkova, Yu. A., & Khvorost, O. P. (2014). Vyvchennia elementnoho skladu miaty pertsevoi (Mentha piperita) [Study of the composition of peppermint (Mentha piperita) elements]. Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky [Current issues in pharmacy and medicine: science and practice], 3, 49–51. [in Ukrainian]
Mishchenko, L. T., Dunich, A. A., Dashchenko, A. V., & Molchanets, O. V. (2014). Vidovoe raznoobrazie virusov, porazhayushchikh rasteniya roda Mentha [Species diversity of viruses infecting plants of Mentha genus]. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Estestvennye nauki [University proceedings. Volga region. Natural Sciences], 2, 29–45. [in Russian]
Senchuhova, N. A. (2003). Virusni khvoroby osnovnykh efirooliinykh kultur Krymskoho rehionu [Viral diseases of staple essential oil crops of Crimea] (Cand. Biol. Sci. Diss.). Taras Shevchenko National University of Kyiv, Kyiv, Ukraine. [in Ukrainian]
Yegorova, N. A., Stavtseva, I. V., Yakimova, O. V., Kamenyok, L. I., & Krivochatko, A. G. (2014). Rol’ nekotorykh faktorov v protsesse induktsii kallusogeneza in vitro u efiromaslichnykh rasteniy [Role of some factors in the process of callusogenesis induction in vitro in essential oil plants]. Faktory eksperymentalnoi evoliutsii orhanizmiv [Factors in Experimental Evolution of Organisms], 15, 63–67. [in Russian]
Kushnir, P. H., & Sarnatska, V. V. (2005). Mikroklonalne rozmnozhennia roslyn: teoriia i praktyka [Microclonal propagation of plants: theory and practice]. Kyiv: Naukova dumka. [in Ukrainian]
Melnychuk, M. D., Novak, T. V. & Kunakh, V. A. (2003). Biotekhnolohiia roslyn [Plant biotechnology]. Kyiv: Polihrafkonsaltynh. [in Ukrainian]
Bugara, I. A. (2013). Klonal’noe mikrorazmnozhenie i ozdorovlenie Mentha piperita L. in vitro [Clonal micropropagation and recovery of Mentha piperita L. in vitro]. Uchenye zapiski Tavricheskogo natsional’nogo universiteta im. V. I. Vernadskogo. Seriya “Biologiya, khimiya”. [Scientific Notes of Taurida V. I. Vernadsky National University. Series: Biology, chemistry], 26(1), 10–15. [in Ukrainian]
Buhara, I. O. (2006). Indukovanyi morfohenez i klonalne mikrorozmnozhennia perspektyvnykh sortiv miaty [Induced morphogenesis and clonal micropropagation of perspective mint varieties]. (Extended Abstract of Cand. Biol. Sci. Diss.). Nikita Botanical Gardens – National Scientific Centre, Yalta, Ukraine. [in Ukrainian]
Bugaenko, L. A., & Shilo, N. P. (2012). Poliploidiya i mezhvidovaya gibridizatsiya u myaty [Polyploidy and interspecific hybridization in mint]. Simferopol: Biznes-Inform. [in Russian]
Sujana, P., & Naidu, C. V. (2011). Indirect plant regeneration from leaf explants of Mentha piperita L. – an important multipurpose medicinal plant. Journal of Phytology, 3(5), 19–22.
Mehta, J, Naruka, R., Sain, M., Dwivedi, A., Sharma, D., & Mirza, J. (2012). An efficient protocol for clonal micropropagation of Mentha piperita L. (Peppermint). Asian Journal of Plant Science and Research, 2(4), 518–523.
Santoro, M. V., Nievasv, F., Zygadlo, J., Giordano, W., & Banchio, E. (2013). Effects of growth regulators on biomass and the production of secondary metabolites in peppermint (Mentha piperita) micropropagated in vitro. American Journal of Plant Sciences, 4, 49–55.
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