DOI: https://doi.org/10.21498/2518-1017.4(33).2016.88666

Allelic status of key genes of carotenogenesis on DNA-markers in maize lines and their hybrids

Ю. О. Гончаров, Т. М. Сатарова, Б. В. Дзюбецький, В. Ю. Черчель

Abstract


Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids.

Methods. DNA isolation, PCR, gel electrophoresis.

Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hyb­rids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activi­ty of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain.

Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly.


Keywords


allele; molecular genetic markers; carotenoge­nesis; maize; line; hybrid

References


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GOST Style Citations


Сатарова Т. Н. Кукуруза: биотехнологические и селекционные аспекты гаплоидии : монография / Т. Н. Сатарова, В. Ю. Черчель, А. В. Черенков. – Днепропетровск : Новая идеология, 2013. – 552 с.

Рослинництво України. Статистичний збірник за 2015 рік / Державна служба статистики України. – К., 2016. – 180 с.

Generation of transgenic maize with enhanced provitamin A content / M. Aluru, Y. Xu, R. Guo [et al.] // J. Exp. Bot. – 2008. – Vol. 59, Iss. 13. – P. 3551–3562. doi: 10.1093/jxb/ern212

Natural variation in the sequence of PSY` and frequency of favorable polymorphism among tropical and temperate maize germplasm / Z. Fu, Y. Chai, Y. Zhou [et al.] // Theor. Appl. Genet. – 2013. – Vol. 126, Iss. 4. – P. 923–935. doi: 10.1007/s00122-012-2026-0

Exploitation of natural variability in maize for β-carotene content using HPLC and gene specific markers / T. Safawo, N. Senthil, M. Raveendran [et al.] // Electron. J. Plant Breed. – 2010. – Vol. 1, Iss. 4. – P. 548–555.

ZmcrtRB3 encodes a carotenoid hydroxylase that affects the accumulation of α-carotene in maize kernel / Y. Zhou, Y. Han, Z. Li [et al.] // J. Integr. Plant Biol. – 2012. – Vol. 54, No. 4. – P. 260–269. doi: 10.1111/j.1744-7909.2012.01106.x

Marker-trait association analysis of functional gene markers for provitamin A level across diverse tropical yellow maize inbred lines / G. Azmach, M. Gedil, A. Menkir, C. Spillane // BMC Plant Biol. – 2013. – Vol. 13. – P. 227. doi: 10.1186/1471-2229-13-227

Development of β-carotene rich maize hybrids through marker-assisted introgression of β-carotene hydroxylase allele / V. Muthusamy, F. Hossain, N. Thirunavukkarasu [et al.] // PLoS ONE. – 2015. – Vol. 9, No. 12. – e113583. doi: 10.1371/journal.pone.0113583

Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme / V. Muthusamy, F. Hossain, N. Thirunavukkarasu [et al.] // Cogent Food and Agriculture. – 2015. – Vol. 1, Iss. 1. – 1033141. doi: 10.1080/23311932.2015.1033141

Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification / C. E. Harjes, T. R. Rocheford, L. Bai [et al.] // Science. – 2008. – Vol. 319. – P. 330–333. doi: 10.1126/science.1150255

Advances in maize genomics and their value for enhancing genetic gains from breeding / Y. Xu, D. J. Skinner, H. Wu [et al.] // Intl J Plant Genomics. – 2009. – Vol. 2009. – Article ID 957602. doi: 10.1155/2009/957602

Yan J. Association mapping for enhancing maize (Zea mays L.) genetic improvement / J. Yan, M. Warburton, J. Crouch // Crop Science. – 2011. – Vol. 51. – P. 433–449.

 

Introgression of the crtRB1 gene into quality protein maize inbred lines using molecular markers / L. Liu, D. Jeffers, Y. Zhang [et al.] // Mol. Breeding. – 2015. – Vol. 35, Iss. 8. – P. 154. doi: 10.1007/s11032-015-0349-7

 

Screening of maize (Zea mays L.) germplasm for crtRB1-3’TE allele enhancing provitamin A concentration in endosperm / D. B. Sagare, P. Shetti, S. S. Reddy [et al.] // Res. Environ. Life Sci. – 2015. – Vol. 8, No. 4. – P. 673–674.

 

Rare genetic variation at Zea mays crtRB1 increases beta-carotene in maize grain / J. Yan, C. B. Kandianis, C. E. Harjes [et al.] // Nat. Genet. – 2010. – Vol. 42, Iss. 4. – P. 322–327. doi: 10.1038/ng.551.

 

Validation of the effects of molecular marker polymorphisms in LcyE and CrtRB1 on provitamin A concentrations for 26 tropical maize populations / R. Babu, N. P. Rojas, S. Gao [et al.] // Theor. Appl. Genet. – 2013. – Vol. 126, Iss. 2. – P. 389–399. doi: 10.1007/s00122-012-1987-3.

 

Identification of β-carotene rich maize inbreds using PCR-based assay for crtRB1-3’TE allele / D. B. Sagare, P. Shetti, S. S. Reddy [et. al.] // I. J. S. N. – 2015. – Vol. 6, No. 3. – P. 441–443.

 

Murray M. G. Rapid isolation of high-molecular weight plant DNA / M. G. Murray, W. F. Thompson // Nuc. Acids Res. – 1980. – Vol. 8, No. 19. – P. 4321–4325.

 

Genetic variability for kernel β-carotene and utilization of crtRB13’TE gene for biofortification in maize (Zea mays L.) / M. Vignesh, F. Hossain, T. Nepolean [et al.] // Indian J Genet Pl Br. – 2012. – Vol. 72, Iss. 2. – P. 189–194.

 

Sequence variation in 3’UTR region of crtRB1 gene and its effect on β-carotene accumulation in maize kernel / M. Vignesh, T. Nepolean, F. Hossain [et al.] // J. Plant Biochem. Biotechnol. – 2013. – Vol. 22, Iss. 4. – P. 401–408. doi: 10.1007/s13562-012-0168-4







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DOI: 10.21498/2518-1017

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