Symbiotic description for alfalfa (<i>Medicago</i> L.) genotypes and their variability
Keywords:alfalfa, tubers, bacterotherapy, mass, selection
Purpose. To make an assessment of the alfalfa selective material according to the intensity of the nodule-forming process and find the correlation with nitrogenase activity. Identify the best genotypes for use in practical breeding.
Methods. Vegetative, statistical.
Results. Conventional varieties of alfalfa according to the number and size of root nodules were analyzed. The variations in the number of nodules were described. In selections, the amount of medium and large nodules (1 mm and bigger in size) increases and the bacterial mass increases too in comparison with the initial forms. Inoculated plants formed 7.6–44.5% more nodules, in comparison to the reference group. Varieties differ in the number of formed nodules of 1 mm and bigger in size with a variation from 19.3 to 82.9%. The nodules on the root system have specific locations. Interest for further selection related to populations: ‘Unitro’, ‘Dobir № 3 – Spr. 2’, ‘Dobir № 5 – Кs.-2007’, which formed 81.2–99.4% of medium and large nodules and 22.7–27.3% of them were located on the main root. The intensity of the nodule-forming process is determined by the degree of root system development. With the increasing of the root system power, the more nodules are formed on it. The level of nitragenase activity depends on the developmental capacity of the alfalfa root system, total number of nodules in sizes of 1–2 mm and > 2 mm.
Conclusions. It is established that the size of the symbiotic apparatus depends not only on the number of nodules, but on its mass too. The intensity of the nodule-forming process is determined by the capacity of the root system. With the increase in the volume of the root system, as a result, more nodules are formed. High correlation coefficients were found between the level of nitrogenase activity, the root system thickness and the total number of nodules on alfalfa plants, including fractions 1–2 mm, > 2 mm.
Tolkachev, N. Z. (2002). Biotechnological aspects of the coordinated selection – nodule bacteria and leguminous plants. In Mikrobiologiya i biotekhnologiya KHKH stoletiya: materialy Mezhdunarodnoy konferentsii [Microbiology and Biotechnology of the XXI Century: Materials Int. Conf.] (pp. 152–153). May 22–24, 2002, Minsk, Belarus. [in Russian]
Didovich, S. V. (2014) Coordinated selection of Mesorhizobium cicuri and Cicer arietinum L. for the symbiotic nitrogen-fixing potential increasing. In Selektsiia ta henetyka bobovykh kultur: suchasni aspekty ta perspektyvy: tezy Mizhnar. nauk. konf. [Selection and genetics of legumes: modern aspects and perspectives: Abstr. Int. Sci. Conf.] (pp. 241–243). June 23–26, 2014, Odesa, Ukraine. [in Ukrainian]
Spaink, H. P., Kondorosi, A., & Hooykaas, P. (Eds.). (1998). The Rhizobiaceae. Molecular Biology of Model Plant-Associated Bacteria. Dordrecht: Springer. doi: 10.1007/978-94-011-5060-6
Tikhonovich, I. A., & Provorov, N. A. (2011). Agricultural microbiology as the basis for ecologically sustainable agriculture: fundamental and applied aspects. Sel’skokhozyaistvennaya biologiya [Agricultural Biology], 3, 3–9. [in Russian]
Didovich, S. V. (2003). Coordinating selection of alfalfa and nodule bacteria for the symbiotic nitrogen fixation increase. Ahroekolohichnyi zhurnal [Agroecological journal], 2, 43–46. [in Russian]
Kolisnyk, A. V., Kolisnyk, I. V., Fedosiienko, D. V., & Koval, R. I. (2005). Economic and technological aspects of the nitrogen bio-fixation in ecological crop production. Naukovyi visnyk NLTU Ukrainy [Scientific Bulletin of Ukrainian National Forestry University], 15.6, 488–491. [in Ukrainian]
Provorov, N. A., Fedorov, S. N., & Voloshin, M. I. (1988). Genetic variability of the leguminous plants according to symbiotic activity with nodule bacteria. Sbornik nauchnykh trudov po prikladnoy botanike, genetike i selektsii [Proceedings on Applied Botany, Genetics and Breeding], 120, 19–23. [in Russian]
Doev, D. N. (2017). Otsenka bioresursnogo potentsiala lyutserny (Medicago varia Mart.) pri ispol’zovanii mestnykh shtammov kluben’kovykh bakteriy roda Sinorhizobium v usloviyakh vertikal’noy zonal’nosti RSO-Alaniya [Estimation of bioresource potential of alfalfa (Medicago varia Mart.) in combination with Sinorhizobium local nodule bacteria strains under conditions of vertical zonation of RSO-Alania] (Cand. Biol. Sci. Diss.). Gorsky State Agrarian University, Vladikavkaz, Russia. [in Russian]
Pereira, P. A., Miranda, B. D., Attewell, J. R., Kmiecik, K. A., & Bliss, F. A. (1993). Selection for increased nodule number in common bean (Phaseolus vulgaris L.). Plant and Soil, 148(2), 203–209. doi: 10.1007/BF00012858
Kazydub, N. G., Marakaeva, T. V., Zolkin, D. A., & Epanchincev, M. V. (2015). Evaluation of the nodule-forming ability of samples of legumes in the Southern Forest-Steppe of Western Siberia. Vestnik Omskogo gosudarstvennogo agrarnogo universiteta [Bulletin of Omsk State Agrarian University], 1, 23–27. [in Russian]
Nutman, P. S. (1967). Varietal differences in the nodulation of subterranean clover. Austral. J. Agric. Res., 18(3), 381–425. doi: 10.1071/AR9670381
Katkov, V. A., & Shain, S. S. (1990). Selection of perennial grasses for symbiotic nitrogen fixation increasing. Selektsiya i semenovodstvo [Plant Breeding and Seed Production], 2, 60–63. [in Russian]
Chunderova, A. I. (1981). On the genetics of bean-rhizobial symbiosis (review). Sel’skokhozyaistvennaya biologiya [Agricultural Вiology], 16(3), 402–406. [in Russian]
Vozhehova, R. A. (Ed.). (2014). Metodyka polovykh i laboratornykh doslidzhen na zroshuvanykh zemliakh [Methods of field and laboratory studies on irrigated lands]. Kherson: N.p. [in Ukrainian]
Dorosinskiy, L. M. (Ed.). (1981). Metodicheskie rekomendatsii dlya kursov povysheniya kvalifikatsii nauchnykh sotrudnikov po sel’skokhozyaystvennoy mikrobiologii [Methodical recommendations for the advanced training courses for researchers in agricultural microbiology]. Leningrad: N.p. [in Russian]
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