Features of the morphological structure of plants and biochemical properties of introduced and domestic varieties of Cannabis sativa L.





Cannabis sativa L., domestic and introduced varieties, biological and morphological features, cannabinoids (CBD, THC, CBN)


Purpose. Identifying the features of the seasonal growth rhythm, development of the plants, morphological and biological differences in monoecious introduced and domestic varieties of Cannabis sativa L. in the North-east region of Ukraine.

Methods. The research was conducted from 2014 till 2019. The following research methods were employed: field (phenological observations, sampling for laboratory analysis), microscopic (study of the morphological structure of glandular and cystolithic hairs on vegetative and generative organs, determination of the size and density of hair formation), laboratory and analytical (determination of the content of cannabinoid substances by thin layer and gas-liquid chromatography), biomorphological me­thods (analysis of morphological characteristics of plants). Chemical analysis for the content of cannabinoid substan­ces was carried out in the laboratory of the breeding and seed production department of the Institute of Bast Crops of the NAAS of Ukraine (Glukhiv, 2014–2019). The content of neutral substances [cannabinol (CBN), cannabidiol (CBD), tetrahydrocannabinol (THC)] and acids (CBDA, THCA) was determined by using a thin layer chromatography (TLC) and gas-liquid chromatography (GLC) methods. For the study, 11 monoecious varieties of C. sativa: ‘YuSO 1’, ‘YuSO 31’, ‘Hliana’, ‘Viktoriia’, ‘Hlesiia’, ‘Hlukhivskyi 46’, ‘Hlukhivski 51’, ‘Zoloto­niski 15’ and 3 introduced varieties: ‘Fedora 17’, ‘Felina 32’, ‘Futura 77’ (France) which differed among themselves in morphological, biological, genetic, ecological-geographical and economically valuable characteristics were selected.

Results. Biological and morphological characteristics of modern monoecious varieties of C. sativa were investigated depending on the ecological and geographical zones of origin, conditions of the growing season and the stage of ontogenesis. The functional interdependence between the formation of specialized excretory structures and the content of cannabinoid substances in plants of different varieties of C. sa­tiva  was revealed. The following promising varieties of C. sativa in a ration of ((THC + CBN) ≤ CBD) point the possibility to use them as the source material for creating the medicinal varie­ties of plants with a high content of CBD.

Conclusions. Nowadays C. sativa is the main source of phyto material for industrial production of natural CBD. In recent years, high-yielding varieties of C. sativa have been created, which, together with high commercially valuable traits, did not contain psychoactive THC and had an insignificant amount of CBD. The results of the research indicated the features of the seasonal growth rhythm, development of the plants and the morphological and biological features of modern monoecious introduced and domestic varieties of C. sativa in the North-east region of Ukraine. The low correlation between the content of cannabinoid substances and their acids in varieties, which are cha­racterized by the low content of cannabinoid compounds was established. According to the developed own classification (THC + KBN): CBD, varieties with a predominant CBD content ‘Hlukhivskyi 46’, ‘Zolotoniski 15’, ‘Hliana’ which are of significant medicinal value were identified.


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Author Biographies

А. С. Полякова, Oleksandr Dovzhenko Hlukhiv National Pedagogical University

Anastasia Poliakova

Л. М. Горшкова, Oleksandr Dovzhenko Hlukhiv National Pedagogical University

Lidia Horskova

Д. Б. Рахметов, M. M. Hryshko National Botanical Garden, National Academy of Sciences of Ukraine

Dzhamal Rakhmetov


Iseger, T. A., & Bossong, M. G. (2015). A systematic review of the antipsychotic properties of cannabidiol in humans. Schizophr. Res., 162(1–3), 153–161. doi: 10.1016/j.schres.2015.01.033

Horshkova, L. M. (2008). Kannabis [Cannabis]. (Part 1). Hluhiv: RVV GDPU. [in Ukrainian]

Akhtar, M. T., Shaari, K., & Verpoorte, R. (2016). Biotransformation of Tetrahydrocannabinol. Phytochem. Rev., 15(5), 921–934. doi: 10.1007/s11101-015-9438-9

Myhal, M. D. (2011). Biologiia lubianih volokon konopel [Biology of hemp bast fibers].Sumy: Papirus. [in Ukrainian]

Todd, A. R. (1946). Hashish. Experientia, 2(2), 55–60. doi: 10.1007/BF02163886

Lesma, G., Consonni, R., Gambaro, V., Remuzzi, C., Roda, G., Silvani, A., … Visconti, G. L. (2014). Cannabinoid-free Cannabis sativa L. grown in the Po valley: evaluation of fatty acid profile, antioxidant capacity and metabolic content. Nat. Prod. Res., 28(21), 1801–1807. doi: 10.1080/14786419.2014.926354

Burstein, S. (2015). Cannabidiol (CBD) and its analogs: a review of their effects on inflammation. Bioorg. Med. Chem., 23(7), 1377–1385. doi: 10.1016/j.bmc.2015.01.059

Docimo, T., Consonni, R.,Coraggio, I., & Mattana, M. (2013). Early phenylpropanoid biosynthetic steps in Cannabis sativa: link between genes and metabolites. Int. J. Mol. Sci., 14(7), 13626–13644. doi: 10.3390/ijms140713626

Myhal, M. D., Sytnyk, І. M., Laiko, І. M. Stupak, T. I., & Riabenka, L. A. (2007). Methods of examination of varieties of hemp (Cannabis sativa L.) for difference, homogeneity and stability. Okhorona prav na sorty roslyn [Protection of Plant Variety Rights], 1(3), 51–63. [in Ukrainian]

Laiko, I. M. (2001). Features of cannabinoid content assessment of some varieties of monoecious hemp. In Selektsiia, tekhnolohiia vyroshchuvannia i zbyrannia lubianykh kultur [Breeding, technology of growing and harvesting bast crops] (Vol. 2, pp. 46–50). Hlukhiv: N.p. [in Russian]

Metodika fenologicheskikh nablyudeniy v botanicheskikh sa­dakh SSSR [Methods of phenological observations in the botanical gardens of theUSSR]. (1979). Byul. Glav. Botan. Sad.Moscow: Nauka. [in Russian]

Giacoppo, S., Mandolino, G., Galuppo, M., Bramanti, P., & Mazzon, E. (2014). Cannabinoids: new promising agents in the treatment of neurological diseases. Molecules, 19(11), 18781–18816. doi: 10.3390/molecules191118781

Zirpel, B., Stehle, F., & Kayser, O. (2015). Production of D9-tetra­hydrocannabinolic acid from cannabigerolic acid by whole cells of Pichia (Komagataella) pastoris expressing D9-tetra­hydrocannabinolic acid synthase from Cannabis sativa L. Bio­technol. Lett., 37, 1869–1875. doi: 10.1007/s10529-015-1853-x

Kmec, I. L. (2011). Features of localization of cystolitic hairs on vegetative and generative organs of hemp. In Agrarna nauka virobnictvu: zb. derzh. nauk.-prakt. konf. «Novitni tehnologiyi v roslinnictvi» [Agrotechnologies for sustainable production of competitive products: collection of the state scientific-practical conference “The latest technologies in crop production”] (pp. 35–36). Nov. 19, 2011,Bila Tserkva,Ukraine. [in Ukrainian]

Kmec, I. L. (2012). Features of development of glandular hairs of hemp and their connection with cannabinoids and pubescence of vegetative and generative organs. In Ahrotekhnolohii dlia staloho vyrobnytstva konkurentnospromozhnoi produktsii: nauk. prakt. konf. molodykh vchenykh i spetsialistiv [Agrotechnologies for sustainable production of competitive products: science. practice. conf. young scientists and specialists] (pp. 4–6). Nov. 29–30, 2012,Kyiv,Ukraine. [in Ukrainian]

Happyana, N., Agnolet, S., Muntendam, R., Van Dam, A., Schnei­der, B., & Kayser, O. (2013). Analysis of cannabinoids in laser-microdissected trichomes of medicinal Cannabis sativa using LCMS and cryogenic NMR. Phytochemistry, 87, 51–59. doi: 10.1016/j.phytochem.2012.11.001



How to Cite

Полякова, А. С., Горшкова, Л. М., & Рахметов, Д. Б. (2020). Features of the morphological structure of plants and biochemical properties of introduced and domestic varieties of Cannabis sativa L. Plant Varieties Studying and Protection, 16(4). https://doi.org/10.21498/2518-1017.16.4.2020.224051