Oxidizing and antioxidant processes in wheat plants infected by Septoria tritici Rob
Keywords:wheat, Septoria tritici Rob., ROS homeostasis, resistance, antioxidant enzymes
Purpose. Based on the study of oxidative and antioxidant processes in wheat plants (Triticum aestivum L.) in the earing phase at the infection by Septoria tritici Rob., identify the varietal features of changes in the level of hydrogen peroxide, the intensity of lipid peroxidation and the activity of antioxidant enzymes for development of biochemical methods for selection of disease-resistant plants.
Methods. Field, spectrophotometric methods of biochemical characteristic determination, comparison, generalization. Statistical analysis of research results was carried out using the program Libre Office Calc (GNU Lesser General Public Licensev3).
Results. Changes in the content of hydrogen peroxide, malondialdehyde and the activity of catalase, superoxide dismutase, peroxidase in wheat plants infected by S. tritici at the earing phase were determined. The presence of varietal features of changes in the oxidative and antioxidant processes of wheat plant cells upon S. tritici infection were detected. It was shown that plant response to S. tritici damage in more disease-resistant wheat varieties were characterized by increased or unchanged relative to the control the content of malondialdehyde and peroxidase activity.
Conclusions. The obtained results will expand the knowledge about the mechanisms of maintaining ROS homeostasis in wheat plants infected by S. tritici and allow to identify biochemical reactions of wheat plants in response to infection, which can be used in the future for the development of biochemical methods for identification of disease-resistant varieties.
Leonov, O. Yu., Petrenkova, V. P., Luchnaya, I. S., Suvorova, K. Yu., & Chugayev, S. V. (2016). Wheat diseases common in Ukraine: harmfulness, genetic control and effectiveness of breeding for resistance. Plant Breeding and Seed Production, 109, 53–92. doi: 10.30835/2413-7510.2016.74196 [In Ukrainian]
Lytvynenko, М. А. (2016). 100-year history of the development of winter wheat breeding programs. Plant Varieties Studying and Protection, 2, 75–82. doi: 10.21498/2518-1017.2(31).2016.70324 [In Ukrainian]
Dumanović, J., Nepovimova, E., Natić, M., Kuča, K., & Jaćević, V. (2021). The significance of reactive oxygen species and antioxidant defense system in plants: a concise overview. Frontiers in Plant Science, 11, Article 552969. doi: 10.3389/fpls.2020.552969
Huang, H., Ullah, F., Zhou, D. X., Yi, M., & Zhao, Y. (2019). Mechanisms of ROS regulation of plant development and stress responses. Frontiers in Plant Science, 10, Article 800. doi: 10.3389/fpls.2019.00800
Zhang, Z., Chen, Y., Li, B., Chen, T., & Tian, S. (2020). Reactive oxygen species: A generalist in regulating development and pathogenicity of phytopathogenic fungi. Computational and Structural Biotechnology, 18, 3344–3349. doi: 10.1016/j.csbj.2020.10.024
Smirnoff, N., & Arnaud, D. (2019). Hydrogen peroxide metabolism and functions in plants. New Phytologist, 221(3), 1197–1214. doi: 10.1111/nph.15488
Hong, J. K., Kang, S. R., Kim, Y. H., Yoon, D. J., Kim, D. H., Kim, H. J., … Kim, Y. S. (2013). Hydrogen peroxide- and nitric oxide-mediated disease control of bacterial wilt in tomato plants. The Plant Pathology Journal, 29(4), 386–396. doi: 10.5423/PPJ.OA.04.2013.0043
Shetty, N. P., Kristensen, B. K., Newman, M.-A., Møller, K., Gregersen, P. L., & Jørgensen, H. J. L. (2003). Association of hydrogen peroxide with restriction of Septoria tritici in resistant wheat. Physiological and Molecular Plant Pathology, 62(6), 333–346. doi: 10.1016/S0885-5765(03)00079-1
Mihailova, G., Stoyanova, Z., Rodeva, R., Bankina, B., Bimšteine, G., & Georgieva, K. (2019). Physiological changes in winter wheat genotypes in response to the Zymoseptoria tritici infection. Photosynthetica, 57(2), 428–437. doi: 10.32615/ps.2019.054
Koch, K. G., Chapman, K., Louis, J., Heng-Moss, T., & Sarath, G. (2016). Physiological changes in winter wheat genotypes in response to the Zymoseptoria tritici infection. Photosynthetica, 7, Article 1363. doi: 10.3389/fpls.2016.01363
Nadarajah, K. K. (2020). ROS homeostasis in abiotic stress tolerance in plants. International Journal of Molecular Sciences, 21(15), Article 5208. doi: 10.3390/ijms21155208
López-Cruz, J., Óscar, C.-S., Emma, F.-C., Pilar, G.-A., & Carmen, G.-B. (2016). Absence of Cu–Zn superoxide dismutase BCSOD1 reduces Botrytis cinerea virulence in Arabidopsis and tomato plants, revealing interplay among reactive oxygen species, callose and signalling pathways. Molecular Plant Pathology, 18(1), 16–31. doi: 10.1111/mpp.12370
Mishchenko, L., Nazarov, T., Dunich, A., Mishchenko, I., Ryshchakova, O., Motsnyi, I., Dashchenko, A., Bezkrovna, L., Fanin, Y., Molodchenkova, O., & Smertenko, A. (2021). Impact of wheat streak mosaic virus on peroxisome proliferation, redox reactions, and resistance responses in wheat. International Journal of Molecular Sciences, 22(19), Article 10218. doi: 10.3390/ijms221910218
Das, K., & Roychoudhury, A. (2014). Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science, 2, Article 53. doi: 10.3389/fenvs.2014.00053
Diani, Z., Ouarraqi, E. M., Aissam, S., Hsissou, D., & Modafar, C. E. (2009). Induction of early oxidative events in soft wheat leaves inoculated with Septoria tritici and their relationship to resistance of Moroccan cultivars. International Journal of Agriculture and Biology, 11(4), 351–359.
Babaiants, O. V., & Babaiants, L. T. (2014). Osnovy selektsii i metodologii otsenok ustoychivosti pshenitsy k vozbuditelyam bolezney [Bases of breeding and methodology of assessments of wheat resistance to pathogens]. Odesa: VМV. [In Russian]
Sagisaka, S. (1976). The Occurrence of Peroxide in a Perennial Plant, Populus gelrica. Plant Physiology, 57(2), 308–309. doi: 10.1104/pp.57.2.308
Merzlyak, M. N., Pogosyan, S. I., Yuferova, S. G., & Shevyreva, V. A. (1978). Using of 2-thiobarbituric acid in the study of lipid peroxidation in plant tissues. Biological Sciences, 9, 86–94. [In Russian]
Ridge, I., & Osborne, D. J. (1970). Hydroxyproline and peroxidases in cell wall of Pisum sativum: regulation by ethylene. Journal of Experimental Botany, 21(4), 843–856. doi: 10.1093/jxb/21.4.843
Korolyuk, M. A., Torev, V. M., & Mayorova, I. G. (1988). Determination of catalase activity. Laboratory Work, 1, 16–18. [In Russian]
Chevari, S., Chaba, I., & Szekely, J. (1985). The role of superoxide dismutase in oxidative cell processes and a method for its determination in biological materials. Laboratory Work, 11, 678–681. [In Russian]
Lowry, O. H., Rosebrough, N. I., Farr, A. L., & Randall, R. J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275. doi: 10.1016/S0021-9258(19)52451-6
Thakur, M., & Sohal, B. S. (2013). Role of elicitors in inducing resistance in plants against pathogen Infection: a review. International Scholarly Research Notices, 2013, Article 762412. doi: 10.1155/2013/762412
Orozco-Cárdenas, M. L., Narváez-Vásquez, J., & Ryan, C. A. (2001). Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. The Plant Cell, 13(1), 179–192. doi: 10.2307/3871162
Poudel, A., Navathe, S., Chand, R., Mishra, V. K., Singh, P. K., & Joshi, A. K. (2019). Hydrogen peroxide prompted lignifications affects pathogenicity of hemi- biotrophic pathogen Bipolaris sorokiniana to wheat. The Plant Pathology Journal, 35(4), 287–300. doi: 10.5423/PPJ.OA.09.2018.0180
Molodchenkova, О. О. (2005). Influence of salicylic acid and Fusarium graminearum on catalase activity, content of H2O2 and endogenous salicylic acid in wheat seedlings. Physiology and Biochemistry of Cultivated Plants, 37(1), 37–43. [In Ukrainian]
El-Beltagi, H. S., & Mohamed, H. I. (2013). Reactive oxygen species, lipid peroxidation and antioxidative defense mechanism. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 44–57. doi: 10.15835/nbha4118929
Yang, F., Melo-Braga, M. N., Larsen, M. R., Jørgensen, H. J. L., & Palmisano, G. (2013). Battle through signaling between wheat and the fungal pathogen Septoria tritici revealed by proteomics and phosphoproteomics. Molecular & Cellular Proteomics, 12(9), 2497–2508. doi: 10.1074/mcp.M113.027532
Sofo, A., Scopa, A., Nuzzaci, M., & Vitti, A. (2015). Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. International Journal of Molecular Sciences, 16(6), 13561–13578. doi: 10.3390/ijms160613561
Goodwin, P. H., Li, J., & Jin, S. (2001). A catalase gene of Сolletotrichum gloeosporioides f. sp. malvae is highly expressed duringthe necrotriphic phase of infection of round-leaved mallow Malva pusilla. FEMS Microbiology Letters, 202(1), 103–107. doi: 10.1111/j.1574-6968.2001.tb10787.x
Mhamdi, A., Queval, G., Chaouch, S., Vanderauwera, S., Van Breusegem, F., & Noctor, G. (2010). Catalase functionin plants: a focus on Arabidopsis mutants as stress–mimic models. Journal of Experimental Botany, 61(15), 4197–4220. doi: 10.1093/jxb/erq282
Almagro, L., Gómez Ros, L. V., Belchi-Navarro, S., Bru, R., Ros Barceló, A., & Pedreño, M. A. (2008). Class III peroxidases in plant defence reactions. Journal of Experimental Botany, 60(2), 377–390. doi: 10.1093/jxb/ern277
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