Biochemical composition of seeds from different pea varieties
DOI:
https://doi.org/10.21498/2518-1017.20.2.2024.304094Keywords:
pea, seed quality, protein, vicilin, legume, flavonoids, anti-nutritional factorsAbstract
Purpose. To study the biochemical parameters characterising seed quality in pea varieties of different morphotypes for the selection of genotypes with improved nutritional properties. Methods. Seeds of 37 different morphotypes [leafy, leafless, heterophillous (chameleon)] of domestic and foreign pea varieties were studied. Standard and laboratory developed methods of biochemical analysis of plants (Kjeldahl method, spectrophotometric methods, electrophoresis) were used. The statistical analysis of the research results was carried out using the software LibreOffice Calc (GNU Lesser General Public License v3) and the image analysis software Imagel. Results. The presence of varietal differences in the biochemical parameters studied related to seed quality (protein content, flavonoids, lipoxygenase activity, trypsin inhibitor, lectins), the content of the main fractions of the protein complex (legumin and vicilin) and their ratio in seeds of different morphotypes was established. The electrophoretic and amino acid analyses revealed varietal differences (in the relative content of certain protein components in the electropherogram, the presence/absence of some components in the electrophoretic spectra of vicilin and legumin, and their amino acid composition) that affect the nutritional value of pea seeds. Conclusions. The application of the biochemical criteria studied makes it possible to select varieties of food peas with specific technological parameters.
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Copyright (c) 2024 O. O. Molodchenkova, S. V. Koblai, P. S. Tykhonov, L. Ya. Bezkrovna, O. V. Ryshchakova, Yu. A. Levitsky, I. A. Untilova
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