DOI: https://doi.org/10.21498/2518-1017.15.4.2019.189295

The influence of genes which responsible for leaf shape and lines height on the economic qualities of Helianthus annuus hybrids

К. В. Ведмедєва, Т. В. Махова

Abstract


Purpose. To study the influence of the Dw, Fr, sp genes which control the morphological markers of lines on the economically valuable indicators of the first-generation hybrids.

Methods. Field experiment, morphological description and biometric measurements, statistical analysis.

Results. The analogue lines were crossed by genes responsible for morphological characteristics of the leaf shape sp (spoon-shaped), leaf edge Fr (fringe), stem Dw (dwarf) and the initial selection lines with sterile lines. The cross-bred hybrids were evaluated in the field experiment on the manifestation of marker trait, yield, oil content, weight of 1000 seeds, height, diameter of the basket (head), the number of leaves. Indicators of line hybrids with morphological marker lines were compared with indicators of hybrids with baseline lines without marker features. The recessive allele of the sp gene determines the spoon-like shape of the leaf, with edges bend upward. This sing is not observed in the first-generation hybrids. Fr gene in the dominant state determines the strongly rugged edge of the leaf. In hybrids of the first generation, changes in the edge of the leaf plate are also observed. The third sign - dwarf of sunflower is determined by the Dw gene, which in the dominant homozygous state reduces the height of plants to 90–100 cm. In the heterozygous state, plants also have a decrease in height. Three breeding lines were used to create analogue lines according to these three criteria. There were two parent ‘LVO7V’, ‘ZL678V’ and one maternal ‘L06B’. Six maternal sterile lines were used to cross with analogue lines. Hybrids were studied by yield, oil content, weight of 1000 seeds, plant height, head diameter, number of leaves.

Conclusions. A high probability of the negative impact of the dominant allele of the Dw gene (dwarfishness – short stem height) on the hybrids productivity was found. The high probability of a positive effect of the recessive allele of the sp gene (spoon shaped leaf) on the yield, oil content and number of leaves of hybrids with a line carrier of recessive homozygotes has been proved. The moderate and low probabilities of the difference in the level of signs of hybrids with the dominant allele of the Fr gene (fringe of the leaf edge) and hybrids with baseline lines were determined.


Keywords


sunflower; yield; oil content; 1000 seeds weight; hybrids; analogue line

References


da Silva, L. J., de Souza Júnior, R. C., & dos Santos Júnior, H. C. (2017). Seed Multiplication and Maintenance. In F. Lopes da Silva, A. Borém, T. Sediyama, & W. Ludke (Eds.), Soybean Breeding (pp. 413–426). Cham: Springer. doi: 10.1007/978-3-319-57433-2_22

Gus'kov, E. P., & Mashkina, E. V. (1999). Genetic analysis of sunflower mutants induced by nitrosomethylurea in combination with 2,4-dinitrophenol or rifampicin. Russ. J. Gen., 35(7), 797–801.

Fambrini, M., Degl’Innocenti, E., Cionini, G., Pugliesi, C., & Guidi, L. (2010). Mesophyll cell defective1, a mutation that disrupts leaf mesophyll differentiation in sunflower. Photosynthetica, 48(1), 135–142. doi: 10.1007/s11099-010-0018-3

Skaloud, V., & Kovacik, A. (1978). Survey on inheritance of sunflower characters which are conditioned by a small number of genes. Proc. 8th Int. Sunflower Conf. (pp. 490–496). July 24–26, 1978, Minneapolis, MN, USA.

Pugliesi, C., Fambrini, M., Barotti, S., Lenzi, A., & Baroncelli, S. (1995). Inheritance of the “Basilicum Leaf” Mutation in Sunflower (Helianthus annuus L.). J. Hered., 86(1), 76–78. doi: 10.1093/oxfordjournals.jhered.a111535

Gavrilova, V. A., & Anisimova, I. N. (2003). Genetics of cultivated plants. Sunflower. St. Petersburg:VIR. [in Russian]

Enns, H., Dorrell, D. G., Hoes, J. A., & Chubb, W. O. (1970). Sunflower research. Proc. 4th Int. Sunflower Conf. (pp. 162–167). June 23–25, 1970, Memphis, Tennessee, USA.

Miller, J. F., & Hammond, J. J. (1991). Inheritance of reduced height in sunflower. Euphytica, 53(2), 131–136. doi: 10.1007/BF00023793

Best, N. B., Wang, X., Brittsan, S., Dean, E., Helfers, S. J., Homburg, R., … Dilkes, B. P. (2016). Sunflower ‘Sunspot’ is hyposensitive to GA3 and has a missense mutation in the DELLA motif of HaDella1. J. Am. Soc. Hort. Sci., 141(4), 389–394. doi: 10.21273/jashs.141.4.389

Hladni, N., Zorić, M., Terzić, S., Ćurčić, N., Satovic, Z., Perović, D., & Panković, D. (2018). Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm. Euphytica. 214(7), 108. doi: 10.1007/s10681-018-2197-0

Alberio, C., Aguirrezabal, L. A. N., Izquierdo, N. G., Reid, R., Zuil, S., & Zambelli, A. (2018). Effect of genetic background on the stability of sunflower fatty acid composition in different high oleic mutations. J. Sci. Food Agric., 98(11), 4074–4084. doi: 10.1002/jsfa.8924

Escalante, C., & Valverde, R. A. (2019). Morphological and physiological characteristics of endornavirus-infected and endornavirus-free near-isogenic lines of bell pepper (Capsicum annuum). Sci. Hortic., 250, 104–112. doi: 10.1016/j.scienta.2019.02.043

Hui, L. L., Zhao, M., He, J. Q., Hu, Y., Huo, Y., Hao, H., … Fu, A. (2019). A simple and reliable method for creating PCR-detectable mutants in Arabidopsis with the polycistronic tRNA-gRNA CRISPR/Cas9 system. Acta Physiol. Plant. 41(10), 170. doi: 10.1007/s11738-019-2961-3

Gong, L., Li, C. F., Capatana, A., Feng, J., Qi, L., Seiler, G. J., & Jan, Ch.-Ch. (2014). Molecular mapping of three nuclear male sterility mutant genes in cultivated sunflower (Helianthus annuus L.). Mol. Breed., 34(1), 159–166. doi: 10.1007/s11032-014-0026-2

Zhang, H., Zhang, D., Han, S., Zhang, X., & Yu, D. (2011). Identification and gene mapping of a soybean chlorophyll-deficient mutant. Plant Breed., 130(2), 133–138. doi: 10.1111/j.1439-0523.2010.01844.x

Schierholt, A., Becker, H. C., & Ecke, W. (2000). Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.). Theor. Appl. Gen., 101(5–6), 897–901. doi: 10.1007/s001220051559

Vedmedeva, K. V., & Soroka, A. I. (2015). Influence of some mutant genes on certain agronomically important traits in sunflower. Helia, 39(64), 57–70. doi: 10.1515/helia-2015-0013

Dospekhov, B. A. (1985). Metodika polevogo opyta (s osnovami statisticheskoy obrabotki rezul'tatov issledovaniy) [Methods of field experiment (with the basics of statistical processing of research results)]. (5nd ed., rev.). Moscow: Agropromizdat. [in Russian]

Nasinnia oliine. Vyznachannia vmistu olii metodom ekstraktsii v aparati Soksleta: DSTU 7577:2014 [Oilseeds. Determination of oil content by Soxhlet apparabus: State Standard of Ukraine 7577:2014]. (2015). Kyiv: Derzhspozhyvstandart Ukrainy. [in Ukrainian]

Ramos, M. L., Altieri, E., Bulos, M., & Sala, C. A. (2013). Phenotypic characterization, genetic mapping and candidate gene analysis of a source conferring reduced plant height in sunflower. Theor. Appl. Gen., 126(1), 251–263. doi: 10.1007/s00122-012-1978-4


GOST Style Citations


da Silva L. J., de Souza Júnior R. C., dos Santos Júnior H. C. Seed Multiplication and Maintenance. Soybean Breeding / F. Lopes da Silva, A. Borém, T. Sediyama, W. Ludke (eds). Cham : Springer, 2017. P. 413–426. doi: 10.1007/978-3-319-57433-2_22

Gus'kov E. P. Mashkina E. V. Genetic analysis of sunflower mutants induced by nitrosomethylurea in combination with 2,4-dinitrophenol or rifampicin. Russ. J. Gen. 1999. Vol. 35, Iss. 7. P. 797–801.

Fambrini M., Degl’Innocenti E., Cionini G. et al. Mesophyll cell defective1, a mutation that disrupts leaf mesophyll differentiation in sunflower. Photosynthetica. 2010. Vol. 48, Iss. 1. P. 135–142. doi: 10.1007/s11099-010-0018-3

Skaloud V., Kovacik A. Survey on inheritance of sunflower characters which are conditioned by a small number of genes. Proc. 8th Int. Sunflower Conf. (July 24–26, 1978, Minneapolis, MN, USA). Minneapolis, 1978. P. 490–496.

Pugliesi C.,  Fambrini M., Barotti S. et al. Inheritance of the “Basilicum Leaf” Mutation in Sunflower (Helianthus annuus L.). J. Hered. 1995. Vol. 86, Iss. 1. P. 76–78.  doi: 10.1093/oxfordjournals.jhered.a111535

Гаврилова В. А., Анисимова И. Н. Генетика культурных растений. Подсолнечник. Санкт-Петербург : ВИР, 2003. 209 с.

Enns H., Dorrell D. G., Hoes J. A., Chubb W. O. Sunflower research. Proc. 4th Int. Sunflower Conf. (June 23–25, 1970, Memphis, Tennessee, USA). Memphis, 1970. P. 162–167.

Miller J. F., Hammond J. J. Inheritance of reduced height in sunflower. Euphytica. 1991. Vol. 53, Iss. 2. P. 131–136. doi: 10.1007/BF00023793

Best N. B., Wang X., Brittsan S. et al. Sunflower ‘Sunspot’ is hyposensitive to GA3 and has a missense mutation in the DELLA motif of HaDella1. J. Am. Soc. Hort. Sci. 2016. Vol. 141, Iss. 4. P. 389–394.  doi: 10.21273/JASHS.141.4.389

Hladni N., Zorić M., Terzić S. et al. Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm. Euphytica. 2018. Vol. 214, Iss. 7. 108 р. doi: 10.1007/s10681-018-2197-0

Alberio C., Aguirrezabal L. A. N., Izquierdo N. G. et al. Effect of genetic background on the stability of sunflower fatty acid composition in different high oleic mutations. J. Sci. Food Agric. 2018. Vol. 98, Iss. 11. P. 4074–4084. doi: 10.1002/jsfa.8924

Escalante C., Valverde R. A. Morphological and physiological characteristics of endornavirus-infected and endornavirus-free near-isogenic lines of bell pepper (Capsicum annuum).  Sci. Hortic. 2019. Vol. 250. P. 104–112. doi: 10.1016/j.scienta.2019.02.043

Hui L. L., Zhao M., He J. Q. et al. A simple and reliable method for creating PCR-detectable mutants in Arabidopsis with the polycistronic tRNA-gRNA CRISPR/Cas9 system. Acta Physiol. Plant. 2019. Vol. 41, Iss. 10. P. 170. doi: 10.1007/s11738-019-2961-3

Gong L., Li C. F., Capatana A. et al. Molecular mapping of three nuclear male sterility mutant genes in cultivated sunflower (Helianthus annuus L.). Mol. Breed. 2014. Vol. 34, Iss. 1. P. 159–166. doi: 10.1007/s11032-014-0026-2

Zhang H., Zhang D., Han S. et al. Identification and gene mapping of a soybean chlorophyll-deficient mutant. Plant Breed. 2011. Vol. 130, Iss. 2. P. 133–138. doi: 10.1111/j.1439-0523.2010.01844.x

Schierholt A., Becker H. C., Ecke W. Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.). Theor. Appl. Gen. 2000. Vol. 101, Iss. 5–6. P. 897–901. doi: 10.1007/s001220051559

Vedmedeva K. V., Soroka A. I. Influence of some mutant genes on certain agronomically important traits in sunflower. Helia. 2015. Vol. 39, Iss. 64. P. 57–70. doi: 10.1515/helia-2015-0013

Доспехов Б. А. Методика полевого опыта (с основами статистической обработки результатов исследований). 5-е изд., доп. и перераб. Москва : Агропромиздат, 1985. 351 с.

Насіння олійне. Визначання вмісту олії методом екстракції в апараті Сокслета : ДСТУ 7577:2014. [Чинний від 2015-05-01]. Київ : Держспоживстандарт України, 2015. 6 с.

Ramos M. L., Altieri E., Bulos M., Sala C. A. Phenotypic characterization, genetic mapping and candidate gene analysis of a source conferring reduced plant height in sunflower. Theor. Appl. Gen. 2013. Vol. 126, Iss. 1, P. 251–263. doi: 10.1007/s00122-012-1978-4







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DOI: 10.21498/2518-1017

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