Auxin activity of piсloram in callusogensis- and morphogenesis of apple (Malus do- mestica borkh.) anthers.

Authors

  • В. Є. Джафарова Всеросійський НДІ селекції плодових культур, Russian Federation

DOI:

https://doi.org/10.21498/2518-1017.1(18).2013.58700

Keywords:

пильники яблуні, ауксинова активність, піклорам, калюсні тканини.

Abstract

Callusogensis and morphogenesis of anthers of apple scab resistant varieties have been studied, with picloram applied. Auxin activity of this preparation has been revealed as in the darkness so in the light. The callusogenesis efficiency of studied varieties varied from 26,0 to 83,3% subject to the concentration of picloram in the darkness and from 11,3 to 74,9% in the conditions of low light. We consider 4 mg/l in the medium to be an optimal level of piloram concentration so far as this concentration provides the best callusogenesis indices of the tested apple varieties. The callus formed in the darkness in the medium with picloram is knobby and dense of white or milky color. The callus formed in the light is intensively green or greenishwhite, denser, with distinctly outlined meristem hearths. Morphological characters of calluses obtained in the medium of picloram are stable over years. The roots of Yubiley Moskvy variety have been induced under auxin:cytokinin ratio 1 : 2 (picloram : BAP, cytokinin). The roots of Svezhest cultivar were obtained under auxin : cytokinin ratio 3:9 (picloram : BAP, kinetin). Formation of four buds was recored in Orlovskoye polesie variety under auxin : cytokinin ratio 1:20.

Downloads

Download data is not yet available.

Author Biography

В. Є. Джафарова, Всеросійський НДІ селекції плодових культур

V.E. Dzafarova

References

Гапоненко, А.К. Перспективы использования культуры клеток растений в селекции / А.К. Гапоненко // Успехи современной генетики. – М.: Наука, 1987. – Вып. 14. – С. 64–74.

Жуков, О.С. Методические рекомендации по получению растений – регенерантов плодовых пород в культуре пыльников / О.С. Жуков, О.Я. Олейникова, Н.И. Савельев. – Мичуринск, 1994. – 36 с.

Шевелуха, В.С. Сельскохозяйственная биотехнология / В.С. Шевелуха, Е.А. Калашникова, С.В. Дегтярев [и др.]. – М.: Высш. шк., 1998. – 416 с.

Kefford, N.P., Caso, O.N. A potent auxin with unique chemical structure – 4 – amino -3,5,6 – trichloropicolinic acid. / N.P. Kefford, O.N. Caso. // Bot. Gaz. – 1966. – Vol. 127. – N 2/3. – C. 159–163.

Гамбург, К.З. Ауксины в культурах тканей и клеток растений / К.З. Гамбург, Н.И. Рекославская, С.Г. Швецов. – Новосибирск: Наука, 1990. 243 с.

Калинин, Ф.Л. Физиология и биохимия культурных растений / Ф.Л. Калинин, В.К. Мусияка. – 1974. – Т. 6, В. 4. – С. 365.

Dulieu, H., Vallee, J. – C., Martin, G. Quelques effects de I’acide 4 – amino – 3,5,6 – trichloropicolinique (piclorame) sur les tissus de Nicotiana tabacum var. Xanthi n. c. en. culture in vitro. / H. Dulieu, J. – C. Vallee, G. Martin // C.R. Acad. Sci. – 1972. – Vol. D. 274. – No18. – P. 2574–2577.

Мусияка, В.К. Активизация деления и растяжения клеток культуры ткани табака в присутствии 4 – амино – 3,5,6 – трихлорпиколиновой кислоты / В.К. Мусияка, Ф.Л. Калинин // Физиология и биохимия культурных растений. – 1975. – Т. 7., No 5. – С. 486–492.

Vian, W.E. The effectiveness of picloram as an auxin source compared to 2,4–D in wheat callus culture medium / W.E. Vian. // Agron. Abstr. – 1976. – P. 65.

Phillips, G.C. Effects of picloram and other auxins on onion tissue cultures / G.C. Phillips, K.J. Luteyn. // J. Amer. Soc. Hort. Sci. – 1983. – Vol. 108., No 6. – P. 948–953.

Чернова, Л.К. Сравнение дифференцирующего воздействия 2,4–Д и 4 – амино – 3,5,6 – трихлорпиколиновой кислоты на ткани бобовых и злаковых растений / Л.К. Чернова, М.Н. Прохоров, Б.В. Филин–Колдаков // Физиология растений. – 1975. – Т. 22, No 2. – С. 170–175.

Mok, M.G. Genotipic responses to auxins in tissue cultures of Phaseolus / M.G. Mok, D. W. S. Mok // Physiol. Plant. – 1977. – V. 40, No 4. – P. 261–264.

Collins, G.B. Use of 4 – amino – 3,5,6, - trichloropicolinic acid as an auxin source in plant tissue cultures / G.B. Collins, W.E. Vian, G.C. Phillips // Crop Sci. – 1978. – V. 18, No 2. – P. 286–288.

Хохлов, В.С. Гаплоидия и селекция / В.С. Хохлов, В.С. Гришина. – М.: Наука, 1976. – 221 с

Published

2013-02-12

How to Cite

Джафарова, В. Є. (2013). Auxin activity of piсloram in callusogensis- and morphogenesis of apple (Malus do- mestica borkh.) anthers. Plant Varieties Studying and Protection, (1(18), 26–28. https://doi.org/10.21498/2518-1017.1(18).2013.58700

Issue

Section

VARIETY STUDYING AND VARIETY SCIENCE