Determination of the criteria for distinctness, uniformity and stability of new varieties of romaine lettuce <i>Lactuca sativa</i> var. <i>longifolia</i> L.
Keywords:romaine lettuce, head, variety, identification, sign, phenotype, polymorphism, distinctness, uniformity, stability
Purpose. To reveal the peculiarities of determining the criteria of distinctness, uniformity and stability of new varieties of romaine lettuce by identifying the phenotype of Lactuca sativa var. longifolia L. in accordance with international requirements and establish the morphological code formula of the official description, according to which the state registration of the variety was carried out.
Methods. Field identification method – morphological description of qualitative (QL), quantitative (QN) and pseudo-qualitative (PQ) characters, laboratory, statistical. Experimental studies were performed during 2015–2017. Field experiments were conducted in the vegetable crop rotation of the Yakimovska variety research station in the Zaporizhzha region under drip irrigation conditions in accordance with the Research Methodology in Vegetable Growing and Melon Growing and the Lactuca sativa L. seed lettuce varieties for distinctness, uniformity and stability.
Results. The article presents the results of field and laboratory studies on the identification of varieties of romaine lettuce Lactuca sativa var. longifolia L. The varieties of this type form a productive organ head with elongated solid leaves and a distinct central vein, the predominant form in cross section is elliptical, the head height is predominantly more than 1.5 times its diameter. Insofar as the vegetative and generative organs of romen lettuce plants varieties were the subject of morphological description of identification signs with the subsequent establishment of a manifestation code. A comprehensive assessment of new varieties of romain lettuce according to morphobiological and economically valuable characteristics is substantiated. For the grouping of varieties, characteristics that do not vary or slightly vary within the variety were used: seed color, anthocyanin leaf color, the start time for the formation of flowering shoots, resistance to downy mildew (Bremia lactucae): Isolate – Bl:16. These signs are used alone or in combination with others.
Conclusions. It was revealed that new varieties of romaine lettuce differed in their morphological characteristics by one or more identifying signs from the well-known varieties ‘Sovskyi’ and ‘Skarb’, which for the period of research represented a collection of varieties of var. longifolia L. New varieties of romaine lettuce ‘Aivona, ‘Batsio, ‘Viktorinus’, ‘Halateia’, ‘Kvintus’, ‘Klaudius’, ‘Korbana’, ‘Maksymus’, ‘Ovired’, ‘Oktavius’, ‘Rafael’ were uniform and stable, as evidenced by the morphological code formulas that made up the official description of the variety, according to which the state registration of the variety was carried out and it was included in the Register of Plant Varieties of Ukraine.
Leshchuk, N. V. (2017). Naukovo-praktychni aspekty identyfikatsii sortiv Lactuca sativa L. [Scientific and practical aspects of Lactuca sativa L. varieties identification]. Vinnytsia: Nilan-LTD. [in Ukrainian]
Ulianych, O. I., & Ketskalo, V. V. (2011). Salat posivnyi [Lettuce]. Uman: Umanske komun. vyd.-polihr. pidp-vo. [in Ukrainian]
Lebeda, A., & Astley, D. (1999). World genetic resources of Lactuca spp., their taxonomy and biodiversity. In Lebeda A., Křístková E. (Eds.), Eucarpia Leafy Vegetables ’99: Proc. of the EUCARPIA Meeting on Leafy Vegetables Genetics and Breeding (p. 81–94). June 8–10, 1999, Olomouc, Czech Republic.
Smilianets, N. M. (1993). Morfobiolohichni osoblyvosti Lactuca sativa var. longifolia u zviazku iz vprovadzhenniam u kulturu na Ukraini [Morphobiological features of Lactuca sativa var. longifolia in connection with cultivation in Ukraine] (Extended Abstract of Cand. Agric. Sci. Diss.). Central Botanical Garden of the Academy of Sciences of Ukraine, Kyiv, Ukraine. [in Ukrainian]
Leshchuk, N. V. (2016). Applying analysis of variance to determine stability of morphological and value for cultivation and use characteristics of Lactuca sativa var. longifolia L. variety of lettuce ‘Skarb’. Plant Var. Stud. Prot., 1, 12–16. doi: 10.21498/2518-1017.1(30).2016.61706
Derzhavnyi reiestr sortiv roslyn, prydatnykh dlia poshyrennia v Ukraini na 2018 r. [State register of plant varieties suitable for dissemination in Ukraine in 2018]. URL: http://sops.gov.ua/uploads/page/5b5abf4cd2673.pdf [in Ukrainian]
Guidelines for the conduct of tests for distinctness, uniformity and stability. Lettuce (Lactuca sativa L.). Retrieved from http://www.upov.int/edocs/tgdocs/en/tg013.pdf
Kondratenko, S. I., Tkalych, Yu. V., Korniienko, S. I., Horova, T. K., Mytenko, I. M., Bashtan, N. O., … Hart, O. Yu. (2015). Metodyka-klasyfikator provedennia ekspertyzy sortiv roslyn na vidminnist, odnoridnist i stabilnist (VOS) salatu posivnoho (Lactuca sativa L.) [Methodology-classifier of examination of plant varieties for difference, uniformity and stability (DUS) of lettuce (Lactuca sativa L.)]. Kharkiv: N.p. [in Ukrainian]
Norwood, J. M., Michelmore, R. W., Crute, I. R., & Ingram, D. S. (1983). The inheritance of specific virulence in Bremia lactucae (downy mildew) to match resistance factors 1, 2, 4, 6 and 11 in Lactuca sativa (lettuce). Plant Pathol.,32(2), 177–186. doi: 10.1111/j.1365-3059.1983.tb01317.x
Bowring, J. D. C. (1969). The identification of varieties of lettuce (Lactuca sativa L.). J. Nat. Inst. Agr. Bot., 11, 499–520.
Atlas morfolohichnykh oznak salatu posivnoho Lactuca sativa L. [Atlas of morphological characteristics of lettuce Lactuca sativa L.]. (2010). Kyiv: Feniks. [in Ukrainian]
Bondarenko, H. L., & Yakovenko, K. I. (Eds.). (2001). Metodyka doslidnoi spravy v ovochivnytstvi i bashtannytstvi [Methods of conducting experiments in vegetable and melon growing] (pp. 11–19, 220, 223). (3rd ed., rev.). Kharkiv: Osnova. [in Ukrainian]
Method of conducting examination of varieties of sown lettuce (Lactuca sativa L.) for difference, homogeneity and stability. (2016). In S. O. Tkachyk (Ed.), Metodyka provedennia ekspertyzy sortiv roslyn hrupy ovochevykh, kartopli ta hrybiv na vidminnist, odnoridnist i stabilnist [Methodology of expert examination of plant varieties of vegetable, potato and mushroom groups for distinctness, uniformity and stability] (pp. 726–746). (2nd ed., rev.). Vinnytsia: Nilan-LTD. [in Ukrainian]
Moyseychenko, V. F. (1988). Metodika opytnogo dela v plodovodstve i ovoshchevodstve [Methodology of experimental work in fruit and vegetable growing] (pp. 3–10, 22, 33–39, 105). Kyiv: Vyshcha shkola. [in Russian]
Byuyul, A., & Tsefel, P. (2002). SPSS: iskusstvo obrabotki. Analiz statisticheskikh dannykh i vosstanovlenie skrytykh zakonomernostey [SPSS: Arts of processing. Analysis of statistical data and restoration of hidden patterns]. St. Petersburg: DiaSoftYuP. [in Russian]
Leschuk, N. V., Mazhuha, K. M., Orlenko, N. S., Starychenko, Ye. M., & Shkapenko, Y. A. (2017). Comparative analysis of statistical software products for the qualifying examination of plant varieties suitable for dissemination. Plant Var. Stud. Prot., 13(4), 429–435. doi: 10.21498/2518-1017.13.4.2017.117757 [in Ukrainian]
How to Cite
Copyright (c) 2018 Н. В. Лещук, Н. С. Орленко, Н. В. Симоненко, О. В. Хареба
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Our journal abides by the CREATIVE COMMONS copyright rights and permissions for open access journals.
Authors, who are published in this journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons Attribution License, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.