Стійкість колекційних зразків ячменю озимого проти комплексу біо- та абіотичних стресових чинників у Лісостепу України

V. M. Hudzenko; A. A. Lysenko; T. P. Polishchuk; N. M. Buniak; M. O. Sardak; T. V. Yurchenko; Ye. A. Kuzmenko; L. V. Khudolii; I. V. Kokhovska; Yu. A. Kravchenko

doi:10.21498/2518-1017.22.1.2026.357581

Authors

V. M. Hudzenko Nosivka Plant Breeding and Experimental Station of the V. M. Remeslo Myronivka Institute of Wheat of the NAAS of Ukraine; Ukrainian Institute for Plant Variety Examination, Ukraine https://orcid.org/0000-0002-9738-1203
A. A. Lysenko “Kyiv-Atlantic Ukraine” LLC, Ukraine https://orcid.org/0000-0002-2575-5720
T. P. Polishchuk The V. M. Remeslo Myronivka Institute of Wheat, NAAS of Ukraine, Ukraine https://orcid.org/0000-0001-9358-9181
N. M. Buniak Nosivka Plant Breeding and Experimental Station of the V. M. Remeslo Myronivka Institute of Wheat of the NAAS of Ukraine, Ukraine https://orcid.org/0000-0002-5089-2399
M. O. Sardak Nosivka Plant Breeding and Experimental Station of the V. M. Remeslo Myronivka Institute of Wheat of the NAAS of Ukraine, Ukraine https://orcid.org/0000-0001-9417-3188
T. V. Yurchenko The V. M. Remeslo Myronivka Institute of Wheat, NAAS of Ukraine, Ukraine https://orcid.org/0000-0003-0164-4003
Ye. A. Kuzmenko “Kyiv-Atlantic Ukraine” LLC, Ukraine https://orcid.org/0000-0002-6256-1482
L. V. Khudolii Ukrainian Institute for Plant Variety Examination, Ukraine https://orcid.org/0000-0002-9586-7592
I. V. Kokhovska Ukrainian Institute for Plant Variety Examination, Ukraine https://orcid.org/0000-0002-0491-3996
Yu. A. Kravchenko Ukrainian Institute for Plant Variety Examination, Ukraine https://orcid.org/0000-0001-7561-1023

DOI:

https://doi.org/10.21498/2518-1017.22.1.2026.357581

Keywords:

Hordeum vulgare L., lodging, powdery mildew, spot blotch, stripe blotch, leaf rust, frost tolerance, drought tolerance, GYT biplot

Abstract

Purpose. To identify the genetic sources of resistance/tolerance to the most common biotic and abiotic stress factors in order to incorporate them into winter barley breeding programs in the Forest-Steppe region of Ukraine. Methods. The study was conducted at the V. M. Remeslo Myronivka Institute of Wheat of NAAS in 2018/19, 2020/21, 2021/22. The study material consisted of 74 accessions of various origins. Assessments of resistance/tolerance to biotic and abiotic factors were carried out under field and laboratory conditions in accordance with standardized methods. GYT biplot analysis was then used to characterize the accessions based on a set of traits and their correlation with yield. Results. As a result of the study, genetic sources of resistance/tolerance to biotic and abiotic stresses have been identified. In particular, sources of resistance to: powdery mildew – 14 accessions (‘MYR 13/1’, ‘MYR 12-1’, ‘MYR 12-12’ (UKR) and others), spot blotch – 13 accessions (‘MYR 12-13’, ‘MYR 12-11’ (UKR); ‘Novosadski 737’ (SRB) and others), leaf stripe – 17 accessions (‘L122’, ‘MYR 12-14’ (UKR); ‘Augusta’ (DEU) and others), leaf rust – three accessions (‘Merlo’ (FRA); ‘MYR 12-12’ (UKR); ‘Scarpia’ (DEU)), lodging – 15 accessions (‘Novosadski 525’ (SRB); ‘Naomie’ (DEU); ‘Panda’ (FRA) and others). Two accessions (‘Manitum’ (FRA), ‘MYR 12-13’ (UKR)) were characterized as a sources of a very high relative frost tolerance. High relative frost tolerance was found for 13 accessions. Higher than average relative drought tolerance was identified in five accessions (‘MYR 12-13’, ‘MYR 12-12’, ‘MYR 4787’, ‘MYR 4790’, ‘MYR 12-8’ (UKR)). The optimal combination of yield and complex of other traits according to GYT biplot was found in the accession ‘Titus’ (DEU). Also, as the sources of the complex traits we can characterized the accessions ‘Merlo’, ‘Manitum’ (FRA); ‘MYR 12-11’, ‘MYR 12-9’, ‘Snihova koroleva’ (UKR); ‘Novosadski 737’ (SRB); ‘Scarpia’ (DEU). However, ‘Merlo’ (FRA) needs improvement in terms of relative frost tolerance. Conclusions. The identified genetic sources of individual traits and their combinations should be used in breeding programmes for winter barley, as well as spring barley in terms of relative drought tolerance and resistance to pathogens. When selecting sources for hybridization based on their resistance to specific biotic and abiotic factors, it is important to consider the expression levels of the remaining traits and combine them with other components on the basis of complementary traits.

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References

Jiang, C., Kan, J., Gao, G., Dockter, C., Li, C., Wu, W., Yang, P., & Stein, N. (2025). Barley2035: A decadal vision for barley research and breeding. Molecular Plant, 18(2), 195–218. https://doi.org/10.1016/j.molp.2024.12.009
|

Lehkun, I. B., Sheremet, O. M., Kovtun, I. V., Skvortsova, K. O., & Shcherbina, Z. V. (2025). Creation of new barley genotypes of winter and alternative types of development. Grain Crops, 9(2), 239–248. https://doi.org/10.31867/2523-4544/0384 [In Ukrainian]

Sadok, W., Wiersma, J. J., Steffenson, B. J., Snapp, S. S., & Smith, K. P. (2022). Improving winter barley adaptation to freezing and heat stresses in the U.S. Midwest: bottlenecks and opportunities. Field Crops Research, 286, Article 108635. https://doi.org/10.1016/j.fcr.2022.108635

Tamang, B. G., López, J.R., McCoy, E., Haaning, A., Sallam, A., Steffenson, B. J., Muehlbauer, G. J., Smith, K. P., & Sadok, W. (2021). Association between xylem vasculature size and freezing survival in winter barley. Journal of Agronomy and Crop Science, 208(3), 362–371. https://doi.org/10.1111/jac.12537

Guerra, D., Morcia, C., Badeck, F., Rizza, F., Delbono, S., Francia, E., Milc, J. A., Monostori, I., Galiba, G., Cattivelli, L., & Tondelli, A. (2021). Extensive allele mining discovers novel genetic diversity in the loci controlling frost tolerance in barley. Theoretical and Applied Genetics, 135(2), 553–569. https://doi.org/10.1007/s00122-021-03985-x
|

Liang, X., Hu, G., McDougall, L., Werth, J., Yang, R., Yang, J., Evans, C., & Satterfield, K. (2024). Small stomates and xylem vessels associated with freeze tolerance in winter barley. Journal of Agronomy and Crop Science, 210(4), Article e12737. https://doi.org/10.1111/jac.12737

Wójcik-Jagła, M., Daszkowska-Golec, A., Fiust, A., Kopeć, P., & Rapacz, M. (2021). Identification of the genetic basis of response to de-acclimation in winter barley. International Journal of Molecular Sciences, 22(3), Article 1057. https://doi.org/10.3390/ijms22031057
|

Wójcik-Jagła, M., & Rapacz, M. (2023). Freezing tolerance and tolerance to de-acclimation of European accessions of winter and facultative barley. Scientific Reports, 13(1), Article 19931. https://doi.org/10.1038/s41598-023-47318-y
|

Pravdziva, I. V., Demydov, O. A., Hudzenko, V. M., & Derhachov, O. L. (2020). Evaluation of yield and stability of bread winter wheat genotypes (Triticum aestivum L.) depending on predecessors and sowing dates. Plant Varieties Studying and Protection, 16(3), 291–302. https://doi.org/10.21498/2518-1017.16.3.2020.214923 [in Ukrainian]

Elakhdar, A., Solanki, S., Takahiko, K., Abed, A., Elakhdar, I., Khedr, R., Hamwieh, A., Capo-chichi, L. J. A., Abdelsattar, M., Franckowiak, J. D., & Qualset, C. O. (2022). Barley with improved drought tolerance: challenges and perspectives. Environmental and Experimental Botany, 201, Article 104965. https://doi.org/10.1016/j.envexpbot.2022.104965

Findurová, H., Veselá, B., Panzarová, K., Pytela, J., Trtílek, M., & Klem, K. (2023). Phenotyping drought tolerance and yield performance of barley using a combination of imaging methods. Environmental and Experimental Botany, 209, Article 105314. https://doi.org/10.1016/j.envexpbot.2023.105314

Badr, A., El-Shazly, H. H., Mahdy, M., Schierenbeck, M., Helmi, R. Y., Börner, A., & Youssef, H. M. (2025). GWAS identifies novel loci linked to seedling growth traits in highly diverse barley population under drought stress. Scientific Reports, 15(1), Article 10085. https://doi.org/10.1038/s41598-025-94175-y
|

Song, R., Shi, P., Xiang, L., He, Y., Dong, Y., Miao, Y., & Qi, J. (2024). Evaluation of barley genotypes for drought adaptability: based on stress indices and comprehensive evaluation as criteria. Frontiers in Plant Science, 15, Article 1436872. https://doi.org/10.3389/fpls.2024.1436872
|

Paul, M., Tanskanen, J., Jääskeläinen, M., Chang, W., Dalal, A., Moshelion, M., & Schulman, A. H. (2023). Drought and recovery in barley: key gene networks and retrotransposon response. Frontiers in Plant Science, 14, Article 1193284. https://doi.org/10.3389/fpls.2023.1193284
|

Cai, K., Gao, H., Wu, X., Zhang, S., Han, Z., Chen, X., Zhang, G., & Zeng F. (2019). The ability to regulate transmembrane potassium transport in root is critical for drought tolerance in barley. International Journal of Molecular Sciences, 20(17), Article 4111. https://doi.org/10.3390/ijms20174111
|

Cai, K., Chen, X., Han, Z., Wu, X., Zhang, S., Li, Q., Nazir, M. M., Zhang, G., & Zeng, F. (2020). Screening of worldwide barley collection for drought tolerance: the assessment of various physiological measures as the selection criteria Frontiers in Plant Science, 11, Article 1159. https://doi.org/10.3389/fpls.2020.01159
|

Wang, J., Yao, L., Hao, J., Li, C., Li, B., Meng, Y., Ma, X., Si, E., Yang, K., Zhang, H., Shang, X., & Wang, H. (2024). Growth properties and metabolomic analysis provide insight into drought tolerance in barley (Hordeum vulgare L.). International Journal of Molecular Sciences, 25(13), Article 7224. https://doi.org/10.3390/ijms25137224
|

Habtegebriel, M. H., Feyissa, T., Setotaw, T. A., & Melkie, Y. (2025). Screening of barley (Hordeum vulgare L.) for early seedling growth traits for drought tolerance under polyethylene glycol 6000. Agrosystems, Geosciences & Environment, 8(3), Article e70203. https://doi.org/10.1002/agg2.70203

Frimpong, F., Anokye, M., Windt, C. W., Naz, A. A., Frei, M., Dusschoten, D. van, & Fiorani, F. (2021). Proline-mediated drought tolerance in the barley (Hordeum vulgare L.) isogenic line is associated with lateral root growth at the early seedling stage. Plants, 10(10), Article 2177. https://doi.org/10.3390/plants10102177
|

Niu, Y., Chen, T., Zhao, C., & Zhou, M. (2022). Lodging prevention in cereals: Morphological, biochemical, anatomical traits and their molecular mechanisms, management and breeding strategies. Field Crops Research, 289, Article 108733. https://doi.org/10.1016/j.fcr.2022.108733

Yuan, Z., Rembe, M., Mascher, M., Stein, N., Himmelbach, A., Jayakodi, M., Börner, A., Oldach, K., Jahoor, A., Jensen, J. D., Rudloff, J., Dohrendorf, V.-E., Kuhfus, L. P., Dyrszka, E., Conte, M., Hinz, F., Trouchaud, S., Reif, J. C., & Hanafi, S. E. (2025). High-quality phenotypic and genotypic dataset of barley genebank core collection to unlock untapped genetic diversity. GigaScience, 14, Article 121. https://doi.org/10.1093/gigascience/giae121
|

Jiang, Y., Xue, R., Chang, Y., Cao, D., Liu, B., & Li, Y. (2025). The knockout of Gγ subunit HvGS3 by CRISPR/Cas9 gene editing improves the lodging resistance of barley through dwarfing and stem strengthening. Theoretical and Applied Genetics, 138(3), Article 61. https://doi.org/10.1007/s00122-025-04853-8
|

Retman, S., Melnichuk, F., Kyslykh, T., & Shevchuk, O. (2022). Complex of barley leaf spots in Ukraine. Chemistry Proceedings, 10(1), Article 1. https://doi.org/10.3390/IOCAG2022-12290

Bilovus, H., Terletska, M., Pushchak, V., Vashchyshyn, O., & Prystatska, O. (2022). Evaluation of winter barley cultivars for resistance to leaf fungal diseases and yield in the conditions of the Western Forest-Steppe of Ukraine. Scientic Horizons, 25(1), 60–67. https://doi.org/10.48077/scihor.25(1).2022.60-67

Bilovus, H., Terletska, M., Ilchuk, R., & Yaremko, V. (2023). Discovery of sources of resistance to leaf diseases of winter barley for use in breeding. Foothill and Mountain Agriculture and Stockbreeding, 73(2), 22–35. https://doi.org/10.32636/01308521.2023-(73)-2-2 [in Ukrainian]

Li, X., Jin, C., Yuan, H., Huang, W., Liu, F., Fan, R., Xie, J., & Shen, Q.-H. (2021). The barley powdery mildew effectorsCSEP0139 and CSEP0182 suppress cell death and promote B. graminis fungal virulence in plants. Phytopathology Research, 3(1), Article 7. https://doi.org/10.1186/s42483-021-00084-z

Dreiseitl, A. (2022). Powdery mildew resistance genes in barley varieties bred for human consumption. Agronomy, 12(10), Article 2245. https://doi.org/10.3390/agronomy12102245

Dreiseitl, A. (2025). Major genes for powdery mildew resistance in research and breeding of barley: a few brief narratives and recommendations. Plants, 14(14), Article 2091. https://doi.org/10.3390/plants14142091
|

Visioni, A., Rehman, S., Viash, S. S., Singh, S. P., Vishwakarma, R., Gyawali, S., Al-Abdallat, A. M., & Verma, R. P. S. (2020). Genome wide association mapping of spot blotch resistance at seedling and adult plant stages in barley. Frontiers in Plant Science, 11, Article 642. https://doi.org/10.3389/fpls.2020.00642
|

Bilovus, H., Vashchyshyn, О., & Pristatska, О. (2023). Dark brown spotting of winter barley in the conditions of the Western Forest Steppe. Bulletin of Agricultural Science, 101(12), 45–50. https://doi.org/10.31073/agrovisnyk202312-06 [in Ukrainian]

Kaur, A., Sharma, V. K., Kaur, S., Kaur, J., & Lal, C. (2020). Characterization of barley entries for spot blotch resistance. International Journal of Current Microbiology and Applied Sciences, 9(10), 161–171. https://doi.org/10.20546/ijcmas.2020.910.021

Roy, D., Dinglasan, E., Fowler, R., Platz, G., Lance, R., Synman, L., Franckowiak, J., Hickey, L. T., Voss-Fels, K., & Robinson, H. (2025). Genomic regions associated with spot blotch resistance in elite barley breeding populations. Molecular Breeding, 45(2), Article 16. https://doi.org/10.1007/s11032-025-01537-5
|

Tucker, J. R., Badea, A., Fernando, W. G. D., Hiebert, C. W., Woitas, A. C., & Beattie, A. D. (2024). Genome-wide association study of adult plant resistance to spot blotch in an elite Canadian two-row barley germplasm collection. Plant Pathology, 73(6), 1446–1457. https://doi.org/10.1111/ppa.13896

Tan, Z., Zhang, S., Qu, Y., Kang, S., Fang, S., & Hou, L. (2025). Identification of leaf stripe resistance genes in hulless barley landrace Teliteqingke from Qinghai-Tibet Plateau. International Journal of Molecular Sciences, 26(3), Article 1133. https://doi.org/10.3390/ijms26031133
|

Wang, Y., Hu, Q., Yao, Y., Cui, Y., Bai, Y., An, L., Li, X., Ding, B., Yao, X., & Wu, K. (2025). Transcriptome, miRNA, and degradome sequencing reveal the leaf stripe (Pyrenophora graminea) resistance genes in Tibetan hulless barley. BMC Plant Biology, 25(1), Article 71. https://doi.org/10.1186/s12870-025-06055-2
|

Si, E., Meng, Y., Ma, X., Li, B., Wang, J., Yao, L., Yang, K., Zhang, Y., Shang, X., & Wang, H. (2020). Genome resource for barley leaf stripe pathogen Pyrenophora graminea. Plant Disease, 104(2), 320–322. https://doi.org/10.1094/PDIS-06-19-1179-A
|

Ziems, L. A., Singh, L., Dracatos, P. M., Dieters, M. J., Sanchez-Garcia, M., Amri, A., Verma, R. P. S., Park, R. F., & Singh, D. (2023). Characterization of leaf rust resistance in international barley germplasm using genome-wide association studies. Plants, 12(4), Article 862. https://doi.org/10.3390/plants12040862
|

Singh, D., Ziems, L. A., Sandhu, K. S., Chhetri, M., Sanchez-Garcia, M., Amri, A., Dieters, M., & Park, R. F. (2025). Predictions of genes conferring resistance to Puccinia hordei in an international barley panel using gene-for-gene-based postulations and linked molecular markers. Plants, 14(20), Article 3150. https://doi.org/10.3390/plants14203150
|

Matros, A., Schikora, A., Ordon, F., & Wehner, G. (2023). QTL for induced resistance against leaf rust in barley. Frontiers in Plant Science, 13, Article 1069087. https://doi.org/10.3389/fpls.2022.1069087
|

Dinh, H. X., Singh, D., Gomez de la Cruz, D., Hensel, G., Kumlehn, J., Mascher, M., Stein, N., Perovic, D., Ayliffe, M., Moscou, M. J., Park, R. F., & Pourkheirandish, M. (2022). The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei. Nature Communications, 13(1), Article 2386. https://doi.org/10.1038/s41467-022-29840-1
|

Hudzenko, V. M., Lysenko, A. A., Polishchuk, T. P., Buniak, N. M., Kuzmenko, Ye. A., Yurchenko, T. V., Khudolii, L. V., & Kokhovska, I. V. (2025). Genetic sources of yield and stability for winter barley breeding under conditions of the Ukrainian Forest-Steppe. Plant Varieties Studying and Protection, 21(1), 25–38. https://doi.org/10.21498/2518-1017.21.1.2025.327499 [In Ukrainian]

Petrenkova, V. P., Borovska, I. Yu., Luchna, I. S., Sokol, T. V., Nyska, I. M., Kucherenko, E. Yu., & Kompanets K. V. (2018). Methodology for selecting forms of field crops in terms of resistance to a complex of biotic and abiotic factors. FOP Brovin O. V. [In Ukrainian]

Yurchenko, T., Pykalo, S., Hudzenko, V., Tomashevska, A. (2024). Method of evaluation and selection of breeding material of winter cereals for frost resistance. Environmental Sciences, 53, 205–208. https://doi.org/10.32846/2306-9716/2024.eco.2-53.28

Yan, W., & Frégeau-Reid, J. (2018). Genotype by yield*trait (GYT) biplot: a novel approach for genotype selection based on multiple traits. Scientific Reports, 8(1), Article 8242. https://doi.org/10.1038/s41598-018-26688-8
|

Frutos E., Galindo M. P., & Leiva V. (2014). An interactive biplot implementation in R for modeling genotype-by-environment interaction. Stochastic Environmental Research and Risk Assessment, 28(7), 1629–1641. https://doi.org/10.1007/s00477-013-0821-z

Laidig, F., Feike, T., Klocke, B. Macholdt, J., Miedaner, T., Rentel, D., & Piepho, H. P. (2021). Long-term breeding progress of yield, yield-related, and disease resistance traits in five cereal crops of German variety trials. Theoretical and Applied Genetics, 134(12), 3805–3827. https://doi.org/10.1007/s00122-021-03929-5
|

Kendal, E. (2020). Evaluation of some barley genotypes with genotype by yield*trait (GYT) biplot method. Agriculture and Forestry, 66(2), 137–150. https://doi.org/10.17707/AgricultForest.66.2.13

Welderufael, S., Abay, F., Ayana, A., & Amede, T. (2023). Genotype by trait (GT) and genotype by yield*traits (GYT) analysis of sorghum landraces in Tigray, Northern Ethiopia. Crop Breeding, Genetics and Genomics, 5(2), Article e230002. https://doi.org/10.20900/cbgg20230002

Hudzenko, V. M., Lysenko, A. A., Tsentylo, L. V., Demydov, O. A., Polishchuk, T. P., Khudolii, L. V., Buniak, N. M., Fedorenko, I. V., Fedorenko, M. V, Petrenko, V. V., Yurchenko, T. V., Suddenko, Y. M., Ishchenko, V. A., & Kozelets, H. M. (2023). Genotype by yield × trait (GYT) biplot analysis for the identification of the superior winter and facultative barley breeding lines. Agronomy Research, 21(2), 739–757. https://doi.org/10.15159/AR.23.052